Culture and identification of neonatal rat brain-derived neural stem cells

Figure 1 Development of neural stem cells derived from newborn rats (inverted phase-contrast microscope). A: At 24 h after the primary culture (100×); B: 3 d after the primary culture (100×); C: 5 d after the primary culture (100×); D: 8 d after the primary culture (100×); E: 4 d after 5 passages (×200); F: 5 d after induction of dissociation (×200).

Figure 2 Immunofluorescence staining. A: BrdU immunofluorescence staining (green fluorescence, ×100); B: Nestin immunofluorescence staining (red fluorescence, ×100); C: NF200 + DAPI immunofluorescence staining (green fluorescence refers to NF200, blue fluorescence refers to DAPI, ×200); D: NSE + DAPI immunofluorescence staining (green fluorescence refers to NSE, blue fluorescence refers to DAPI, ×200); E: GFAP + DAPI immunofluorescence staining (green fluorescence refers to GFAP, blue fluorescence refers to DAPI, ×200).