Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

doi:10.4252/wjsc.v2.i6.127

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Dec 26, 2010; 2(6): 127-140
Published online Dec 26, 2010. doi: 10.4252/wjsc.v2.i6.127

Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

Solomon Mamo, Julianna Kobolak, Istvan Borbíró, Tamás Bíró, Istvan Bock, Andras Dinnyes

Solomon Mamo, Julianna Kobolak, Andras Dinnyes, Genetic Reprogramming Group, Agricultural Biotechnology Center, Szent-Gyorgyi Albert ut. 4, H-2100 Gödöllő, Hungary

Solomon Mamo, Lyons Research Farm, University College Dublin, Newcastle, Co Dublin, Ireland

Istvan Borbíró, Tamás Bíró, Department of Physiology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Nagyerdei krt. 98, H-4032 Debrecen, Hungary; Abiol Ltd., Nagyerdei krt. 98, H-4032 Debrecen, Hungary

Istvan Bock, BioTalentum Ltd., Aulich l. 26, Gödöllő, Hungary

Andras Dinnyes, Molecular Animal Biotechnology Laboratory, Szent Istvan University, Páter K. u. 1, H-2103 Gödöllő, Hungary

Author contributions: Mamo S participated in the experimental design, coordinated molecular biology activities, performed real time PCR and microarray analysis, interpretation of the data and was the primary author of the manuscript; Kobolak J conceived the experiment, performed the stem cell culture, Southern blot, and participated in manuscript preparation; Borbíró I performed the Ca²⁺ assay measurement; Bíró T performed Ca²⁺ assay data analysis and participated in manuscript preparation; Bock I performed the real time PCR experiment and data analysis for the various Ca²⁺ receptors; Dinnyes A supervised the study design, execution, analysis, and approved the final version.

Supported by EU FP6 (TEAMOHOLIC “MEXT-CT-2003-509582”, “MEDRAT” LSHG-CT-2006-518240, “CLONET” MRTN-CT-2006-035468), EU FP7 (“PartnErS” PIAPGA-2008-218205; “Plurisys” HEALTH-F4-2009-223485; “RESOLVE” FP7-HEALTH-F4-2008-202047), NKFP_07_1-ES2HEART-HU (OM-00202-2007), Hungarian-Chinese NKTH TET, No. CN-56/2007 and “Plurabit” NKTH TET-09-1-2010-0007

Correspondence to: Andras Dinnyes, PhD, DSc, Professor, Molecular Animal Biotechnology Laboratory, Szent Istvan University, Páter K. u. 1, H-2103 Gödöllő, Hungary. andrasdinnyes@yahoo.com

Telephone: +36-20-5109632 Fax: +36-28-526151

Received: March 24, 2010
Revised: October 5, 2010
Accepted: October 12, 2010
Published online: December 26, 2010

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