3T3-L1细胞中FoxO1基因沉默对高分子量脂联素表达的影响

表1 shRNA-FoxO1序列

名称	序列
NC	F: 5'-TTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTTC-3'
NC	R: 5'-TCGAGAAAAAATTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAA-3'
FoxO1-shRNA-1	F: 5'-TGACTTTGATAACTGGAGTATTCAAGAGATACTCCAGTTATCAAAGTCTTTTTTC-3'
FoxO1-shRNA-1	R: 5'-TCGAGAAAAAAGACTTTGATAACTGGAGTATCTCTTGAATACTCCAGTTATCAAAGTCA-3'
FoxO1-shRNA-2	F: 5'-TGCCCTGGGTCTCACAGCAATTCAAGAGATTGCTGTGAGACCCAGGGCTTTTTTC-3'
FoxO1-shRNA-2	R: 5'-TCGAGAAAAAAGCCCTGGGTCTCACAGCAATCTCTTGAATTGCTGTGAGACCCAGGGCA-3'
FoxO1-shRNA-3	F: 5'-TGGAGAACCTTCTGGATAATTTCAAGAGAATTATCCAGAAGGTTCTCCTTTTTTC-3'
FoxO1-shRNA-3	R: 5'-TCGAGAAAAAAGGAGAACCTTCTGGATAATTCTCTTGAAATTATCCAGAAGGTTCTCCA-3'
FoxO1-shRNA-4	F: 5'-TGAGGATTGAACCAGTATAATTCAAGAGATTATACTGGTTCAATCCTCTTTTTTC-3'
FoxO1-shRNA-4	R: 5'-TCGAGAAAAAAGAGGATTGAACCAGTATAATCTCTTGAATTATACTGGTTCAATCCTCA-3'

F: 上游引物; R: 下游引物.

表2 基因名称与引物序列

基因名称	引物序列
Mus Actin	上游引物: GGCTGTATTCCCCTCCATCG
Mus Actin	下游引物: CAGTTGGTAACAATGCCATGT
Mus FoxO1	上游引物: CTCATCACCAAGGCCATCGAG
Mus FoxO1	下游引物: CCATGGACGCAGCTCTTCTC