Copyright
©The Author(s) 2016.
世界华人消化杂志. 2016-07-08; 24(19): 2974-2981
在线出版 2016-07-08. doi: 10.11569/wcjd.v24.i19.2974
在线出版 2016-07-08. doi: 10.11569/wcjd.v24.i19.2974
SMO siRNA片段 | 增加物 | 目标序列 | 片段 | GC比例(%) |
SMO-RNAi(37303-1) | NM_005631 | TGATGGACACAGAACTCAT | 281.2644 | 42.11 |
SMO-RNAi(37304-1) | NM_005631 | GGAGAAGATCAACCTGTTT | 281.2644 | 42.11 |
SMO-RNAi(37305-1) | NM_005631 | TGACTGTGAGATCAAGAAT | 281.2644 | 36.84 |
Description | Homo sapiens smoothened homolog(Drosophila) (SMO), mRNA |
引物 | 5'端 | 茎 | 环 | 茎 | 3'端 |
SMO-RNAi(37303-1)-a | Ccgg | ccTGATGGACACAGAACTCAT | CTCGAG | ATGAGTTCTGTGTCCATCAGG | TTTTTg |
SMO-RNAi(37303-1)-b | aattcaaaaa | ccTGATGGACACAGAACTCAT | CTCGAG | ATGAGTTCTGTGTCCATCAGG | |
SMO-RNAi(37304-1)-a | Ccgg | gtGGAGAAGATCAACCTGTTT | CTCGAG | AAACAGGTTGATCTTCTCCAC | TTTTTg |
SMO-RNAi(37304-1)-b | aattcaaaaa | gtGGAGAAGATCAACCTGTTT | CTCGAG | AAACAGGTTGATCTTCTCCAC | |
SMO-RNAi(37305-1)-a | Ccgg | ccTGACTGTGAGATCAAGAAT | CTCGAG | ATTCTTGATCTCACAGTCAGG | TTTTTg |
SMO-RNAi(37305-1)-b | aattcaaaaa | ccTGACTGTGAGATCAAGAAT | CTCGAG | ATTCTTGATCTCACAGTCAGG |
引文著录: 迪力夏提•吐尼牙孜, 丁伟, 依马木买买提江•阿布拉, 易超, 苏雅婷, 李海军. SMO基因siRNA慢病毒表达载体的构建及其对胰腺癌细胞SMO基因表达的影响. 世界华人消化杂志 2016; 24(19): 2974-2981