基础研究
Copyright ©The Author(s) 2016.
世界华人消化杂志. 2016-07-08; 24(19): 2974-2981
在线出版 2016-07-08. doi: 10.11569/wcjd.v24.i19.2974
表1 3条SMO siRNA片段信息
SMO siRNA片段增加物目标序列片段GC比例(%)
SMO-RNAi(37303-1)NM_005631TGATGGACACAGAACTCAT281.264442.11
SMO-RNAi(37304-1)NM_005631GGAGAAGATCAACCTGTTT281.264442.11
SMO-RNAi(37305-1)NM_005631TGACTGTGAGATCAAGAAT281.264436.84
DescriptionHomo sapiens smoothened homolog(Drosophila) (SMO), mRNA
表2 SMO siRNA引物信息
引物5'端3'端
SMO-RNAi(37303-1)-aCcggccTGATGGACACAGAACTCATCTCGAGATGAGTTCTGTGTCCATCAGGTTTTTg
SMO-RNAi(37303-1)-baattcaaaaaccTGATGGACACAGAACTCATCTCGAGATGAGTTCTGTGTCCATCAGG
SMO-RNAi(37304-1)-aCcgggtGGAGAAGATCAACCTGTTTCTCGAGAAACAGGTTGATCTTCTCCACTTTTTg
SMO-RNAi(37304-1)-baattcaaaaagtGGAGAAGATCAACCTGTTTCTCGAGAAACAGGTTGATCTTCTCCAC
SMO-RNAi(37305-1)-aCcggccTGACTGTGAGATCAAGAATCTCGAGATTCTTGATCTCACAGTCAGGTTTTTg
SMO-RNAi(37305-1)-baattcaaaaaccTGACTGTGAGATCAAGAATCTCGAGATTCTTGATCTCACAGTCAGG

引文著录: 迪力夏提•吐尼牙孜, 丁伟, 依马木买买提江•阿布拉, 易超, 苏雅婷, 李海军. SMO基因siRNA慢病毒表达载体的构建及其对胰腺癌细胞SMO基因表达的影响. 世界华人消化杂志 2016; 24(19): 2974-2981