Copyright
©The Author(s) 2015.
世界华人消化杂志. 2015-01-08; 23(1): 78-84
在线出版 2015-01-08. doi: 10.11569/wcjd.v23.i1.78
在线出版 2015-01-08. doi: 10.11569/wcjd.v23.i1.78
表1 miRNA芯片检测RF48及RF1中差异表达的miRNA
| MicroRNA | RF48/RF1 | MicroRNA | RF48/RF1 |
| Up regulaiton | Fold change | Down regulaiton | Fold change |
| miRNA-193 | 5.133 | miRNA-455 | 0.065 |
| miRNA-30b | 4.632 | miRNA-196b | 0.187 |
| miRNA-194 | 4.513 | miRNA-744-3p | 0.355 |
| miRNA-551b | 3.866 | miRNA-139-3p | 0.365 |
| miRNA-215 | 3.559 | miRNA-885 | 0.386 |
| miRNA-2101 | 3.359 | miRNA-630 | 0.389 |
| miRNA-301a | 3.305 | miRNA-196a | 0.412 |
| miRNA-33a | 3.205 | miRNA-432 | 0.417 |
| miRNA-192 | 3.038 | miRNA-1227 | 0.447 |
| miRNA-100 | 2.871 | miRNA-223 | 0.451 |
| miRNA-23b | 2.757 | miRNA-30b* | 0.454 |
| miRNA-145 | 2.742 | miRNA-197 | 0.455 |
| miRNA-342 | 2.708 | miRNA-181d | 0.470 |
| hsa-let-7e | 2.405 | miRNA-574 | 0.487 |
| miRNA-192 | 2.189 | hsa-let-7b | 0.499 |
| miRNA-30a | 2.160 | ||
| miRNA-324 | 2.110 | ||
| miRNA-425 | 2.072 | ||
| miRNA-27b | 2.034 | ||
| miRNA-24 | 2.032 | ||
| miRNA-125a | 2.005 |
表2 miRNA-210靶基因GO分析
| 名称 | 基因数 | P值 | 富集倍数 |
| GOTERM_BP_FAT n = 407 | |||
| GO:0042127-regulation of cell proliferation | 29 | 1.85×10-11 | 4.491 |
| GO:0043066-negative regulation of apoptosis | 17 | 2.58×10-8 | 5.853 |
| GO:0043069-negative regulation of programmed cell death | 17 | 3.13×10-8 | 5.771 |
| GO:0060548-negative regulation of cell death | 17 | 3.26×10-8 | 5.755 |
| GO:0051329-interphase of mitotic cell cycle | 10 | 1.48×10-7 | 11.832 |
| GOTERM_CC_FAT n = 31 | |||
| GO:0031967-organelle envelope | 12 | 0.005 | 2.660 |
| GO:0031975-envelope | 12 | 0.005 | 2.652 |
| GO:0031410-cytoplasmic vesicle | 12 | 0.006 | 2.569 |
| GO:0031982-vesicle | 12 | 0.008 | 2.462 |
| GO:0030141-secretory granule | 6 | 0.009 | 4.581 |
| GOTERM_MF_FAT n = 51 | |||
| GO:0004714-transmembrane receptor protein tyrosine kinase activity | 6 | 1.95×10-4 | 11.073 |
| GO:0019899-enzyme binding | 14 | 2.70×10-4 | 3.310 |
| GO:0008134-transcription factor binding | 13 | 8.06×10-4 | 3.133 |
| GO:0004672-protein kinase activity | 14 | 0.001 | 2.857 |
| GO:0043566-structure-specific DNA binding | 7 | 0.001 | 5.969 |
表3 miRNA-210靶基因的KEGG通路分析
| 名称 | 百分比(%) | P值 | 富集倍数 | FDR值 |
| hsa05200: Pathways in cancer | 16.40 | 5×10-9 | 5.167 | 5.769×10-6 |
| hsa05215: Prostate cancer | 9.48 | 3×10-8 | 11.026 | 3.263×10-5 |
| hsa05219: Bladder cancer | 6.90 | 3×10-7 | 16.992 | 0.293 |
| hsa04012: ErbB signaling pathway | 8.62 | 3×10-7 | 10.254 | 0.356×10-3 |
| hsa05212: Pancreatic cancer | 7.76 | 9×10-7 | 11.151 | 0.984×10-3 |
| hsa05213: Endometrial cancer | 6.90 | 10-6 | 13.724 | 0.133×10-2 |
| hsa05220: Chronic myeloid leukemia | 7.76 | 10-6 | 10.705 | 0.135×10-2 |
| hsa05210: Colorectal cancer | 6.90 | 3×10-5 | 8.4962 | 0.034 |
引文著录: 张小静, 冯先玲, 黄勇, 高燕, 曹子一, 黄伟玲, 张媛, 简千贺, 钟锦成, 杨梦婷, 范新民, 金哲. miRNA-210在胃癌转移细胞株中的表达及功能预测. 世界华人消化杂志 2015; 23(1): 78-84