应用二代测序技术筛选压力作用下肝星状细胞的差异表达基因

表1 PCR引物列表

引物名称	序列	扩增大小(bp)
Oas1a	F: 5'-CGGAGGTCATGGAGCAGGAACT-3'	253
	R: 5'-AACACCACCAGGTCAGCGTCT-3'
Mx2	F: 5'- TGCCTGCCATCGCTGTCATC-3'	159
	R: 5'-TGCCTTTCCACTCCTCTCCCT-3'
Apol9a	F: 5'- GCCTCCGACATCCTGAACCTCT-3'	221
	R: 5'-ACACTTGTGCCAGCTTCCTCCA-3'
Lpl	F: 5'- AGGTCAGAGCCAAGAGAAGCAGT-3'	297
	R: 5'-ACCAGCGGAAGTAGGAGTCGTT-3'
Mmp12	F: 5'- CTGGGCAACTGGACACCTCAAC-3'	159
	R: 5'- CCGCACGCTTCATGTCTGGA-3'

F: 上游引物; R: 下游引物.

表2 压力作用肝星状细胞表达上调基因

基因名称	相关描述	差异倍数	FDR
LOC100364191	hCG1994130-like	8.579	1.22×10-18
Tf	Transferrin	9.081	2.11×10-47
Oas1a	2'-5' oligoadenylate synthetase 1A	10.452	8.93×10-4
Mx2	Myxovirus (influenza virus) resistance 2	9.655	0.036
Apol9a	Apolipoprotein L 9a	15.945	3.19×10-5
Irf7	Interferon regulatory factor 7	10.474	2.73×10-24
LOC680329	Immunoglobulin lambda-like polypeptide 5-like	8.161	0.045
Ifit1	Interferon-induced protein with tetratricopeptide repeats 1	17.326	1.06×10-4
Igha	Immunoglobulin heavy chain, alpha	20.910	6.39×10-6
Marco	Macrophage receptor with collagenous structure	11.405	1.05×10-4

差异倍数≥8, FDR<0.05. FDR: 错误发现率.

表3 压力作用肝星状细胞表达下调基因

基因名称	相关描述	差异倍数	FDR
LOC688452	Hypothetical protein LOC688452	0.081	1.67×10-3
Lpl	Lipoprotein lipase	0.110	4.22×10-3
LOC682206	Similar to Zinc finger protein 208	0.089	6.35×10-4
Mmp12	Matrix metallopeptidase 12	0.114	3.51×10-11

Fold Change≤0.125, FDR<0.05. FDR: 错误发现率.