散发性结直肠癌组织病理学特征及微卫星不稳定状态分析400例

表1 5对微卫星引物的位置和序列

引物名称	引物序列	扩增片段(bp)
BAT25F	6FAM-TCGCCTCCAAGAATGTAAGT	chr4: 55598151+55598274
BAT25R	TCTGCATTTTAACTATGGCTC	124
BAT26F	HEX-TGACTACTTTTGACTTCAGCC	chr2: 47641487+47641608
BAT26R	AACCATTCAACATTTTTAACCC	122
APCF(D5S346)	HEX-ACTCACTCTAGTGATAAATCG	chr5: 112213624-112213748
APCR(D5S346)	AGCAGATAAGACAGTATTACTAGTT	125
Mfd26CAF(D17S250)	6FAM-GGAAGAATCAAATAGACAAT	chr17: 37152092-37152243
Mfd26CAR(D17S250)	GCTGGCCATATATATATTTAAACC	152
AFM093xh3F(D2S123)	HEX-AAACAGGATGCCTGCCTTTA	chr2: 51288437+51288647
AFM093xh3R(D2S123)	GGACTTTCCACCTATGGGAC	211

表2 18例MSI表达状态分型

序号	MSI状态
	MLH1	MSH2	MSH6	PSM2	MSI
1	-	+	-	-	MSI-H
2	+	-	-	+	MSI-H
3	-	+	-	+	MSI-H
4	-	-	-	+	MSI-H
5	-	-	-	+	MSI-H
6	+	-	-	+	MSI-H
7	-	-	-	+	MSI-H
8	+	-	-	+	MSI-H
9	-	-	-	+	MSI-H
10	-	+	-	+	MSI-H
11	-	-	+	-	MSI-H
12	+	-	-	+	MSI-H
13	+	-	+	+	MSI-H
14	-	+	-	+	MSI-H
15	-	-	-	+	MSI-H
16	-	-	-	+	MSI-H
17	+	-	-	+	MSI-H
18	-	-	-	+	MSI-H

MSI: 微卫星不稳定性; MSI-H: 高度微卫星不稳定性.