二乙基亚硝胺诱发肝癌前病变大鼠模型的建立

表1 大鼠体质量变化 (mean±SD, n_i = 20)

剂量(mg/kg)	体质量(g)	变化率
0	378.6±3.72	0.00
25	270.5±2.11b	-0.29
50	254.6±2.60bd	-0.33
75	216.4±2.72bd	-0.43
100	156.3±2.51bdfh	-0.59

t-test, vs 0剂量组, ^aP<0.05,

^bP<0.01; vs 25剂量组, ^cP<0.05,

^dP<0.01; vs 50剂量组, ^eP<0.05,

^fP<0.01; vs 75剂量表, ^gP<0.05,

^hP<0.01.

表2 二乙基亚硝胺诱发大鼠肝细胞癌前病变的形态计量 (mean±SD, n_i = 2)

剂量(mg/kg)	时程(log(h))	体积比			异型性指数(AI)	癌变率(P/T)
		汇管区(R1)	肝索(R2) 肝细胞核(R3)
0	1.056905	0.0009	0.3991	0.0009	0.0021	0/2
	2.540830	0.0009	0.4294	0.0010	0.0023	0/2
	2.834675	0.0011	0.5066	0.0015	0.0030	0/2
	3.008345	0.0011	0.5143	0.0017	0.0034	0/2
	3.132067	0.0020	0.5696	0.0019	0.0033	0/2
	3.228246	0.0020	0.5782	0.0022	0.0037	0/2
	3.306939	0.0035	0.4176	0.0016	0.0038	0/2
	3.373537	0.0033	0.4858	0.0019	0.0040	0/2
	3.431267	0.0034	0.5008	0.0021	0.0042	0/2
	3.482216	0.0035	0.5159	0.0031	0.0061	0/2
25	1.056905	0.0009	0.4585	0.0009	0.0020	0/2
	2.540830	0.0010	0.5127	0.0010	0.0020	0/2
	2.834675	0.0013	0.5581	0.0020	0.0035	0/2
	3.008345	0.0018	0.5771	0.0031	0.0053	0/2
	3.132067	0.0023	0.6489	0.0042	0.0064	0/2
	3.228246	0.0033	0.6533	0.0057	0.0087	0/2
	3.306939	0.0035	0.6636	0.0059	0.0090	0/2
	3.373537	0.0039	0.6691	0.0061	0.0092	0/2
	3.431267	0.0041	0.6822	0.0073	0.0108	0/2
	3.482216	0.0043	0.6951	0.0081	0.0117	0/2
50	1.056905	0.0006	0.4726	0.0013	0.0027	0/2
	2.540830	0.0032	0.4205	0.0014	0.0033	0/2
	2.834675	0.0086	0.5293	0.0024	0.0046	0/2
	3.008345	0.0092	0.5913	0.0051	0.0087	0/2
	3.132067	0.0095	0.6802	0.0061	0.0091	0/2
	3.228246	0.0098	0.6743	0.0075	0.0113	0/2
	3.306939	0.0101	0.7007	0.0086	0.0125	0/2
	3.373537	0.0107	0.6377	0.0092	0.0145	0/2
	3.431267	0.0149	0.8235	0.0118	0.0145	0/2
	3.482216	0.0155	0.7986	0.0122	0.0155	1/2
75	1.056905	0.0008	0.5089	0.0015	0.0029	0/2
	2.540830	0.0018	0.5330	0.0019	0.0036	0/2
	2.834675	0.0111	0.6107	0.0054	0.0090	0/2
	3.008345	0.0092	0.6822	0.0071	0.0105	0/2
	3.132067	0.0109	0.7848	0.0106	0.0137	0/2
	3.228246	0.0097	0.7779	0.0133	0.0173	0/2
	3.306939	0.0116	0.8084	0.0146	0.0183	0/2
	3.373537	0.0124	0.7630	0.0164	0.0218	0/2
	3.431267	0.0172	0.8346	0.0178	0.0216	1/2
	3.482216	0.0179	0.8352	0.0184	0.0224	1/2
100	1.056905	0.0010	0.4129	0.0012	0.0030	0/2
	2.540830	0.0011	0.4241	0.0014	0.0032	0/2
	2.834675	0.0043	0.4833	0.0040	0.0083	0/2
	3.008345	0.0073	0.5900	0.0077	0.0131	0/2
	3.132067	0.0119	0.6294	0.0093	0.0150	0/2
	3.228246	0.0162	0.6230	0.0109	0.0178	0/2
	3.306939	0.0188	0.7528	0.0144	0.0195	0/2
	3.373537	0.0190	0.7751	0.0164	0.0216	1/2
	3.431267	0.0217	0.8012	0.0175	0.0224	2/2
	3.482216	0.0224	0.8347	0.0184	0.0225	2/2

表3 二乙基亚硝胺诱发大鼠肝细胞癌前病变的主要时效特征 (mean±SD, n_i = 20)

剂量(mg/kg)	半效时程(h)	可信限(h)	时效方程	R	AUC
0	70347(419Ws)	0-∞	Y = 0.002398+0.438102/(1+10(7.692189-1.587X))	0.9242	0.0064
25	1734(10Ws)	936.5-3211	Y = 0.00177+0.01276/(1+10(6.309572-1.948X))	0.9931	0.0084
50	1536(9Ws)	947.9-2490	Y = 0.002557+0.016193/(1+10(6.336954-1.989X))	0.9935	0.0123
75	1530(9Ws)	889.7-2632	Y = 0.002698+0.026002/(1+10(5.71389-1.794X))	0.9942	0.0165
100	1183(7Ws)	954.9-1466	Y = 0.002487+0.023033/(1+10(6.428716-2.092X))	0.9970	0.0167