MicroRNA在直肠癌中的表达谱

表1 组织总RNA提取的完整性及浓度

组织编号	A260/280	A260/230	浓度(μg/μL)	总量(μg)	样品质量描述
C1	2.09	2.12	0.848	39.3	RNA完好
C2	2.06	2.16	0.606	28.0	RNA完好
N1	2.07	2.07	0.257	11.6	RNA完好
N2	2.06	2.00	0.225	10.1	RNA完好

表2 直肠腺癌与正常直肠黏膜组织表达差异的miRNA

名称	C1	C2	N1	N2	比值	序列
上调
miR-15a	21	28	13	10	2.051	CAGGCCAUAUUGUGCUGCCUCA
miR-18a	2732	5333	705	896	5.039	UAAGGUGCAUCUAGUGCAGAUAG
miR-18a*	116	215	43	28	4.658	ACUGCCCUAAGUGCUCCUUCUGG
miR-21	207	230	70	80	2.903	CAACACCAGUCGAUGGGCUGU
miR-24-2*	138	247	132	54	2.069	UGCCUACUGAGCUGAAACACAG
miR-27a	171	333	128	82	2.406	AGGGCUUAGCUGCUUGUGAGCA
miR-31	2774	610	116	196	10.845	AGGCAAGAUGCUGGCAUAGCU
miR-92a-1*	137	113	41	71	2.230	AGGUUGGGAUCGGUUGCAAUGCU
miR-29b-1*	132	198	55	35	3.649	GCUGGUUUCAUAUGGUGGUUUAGA
miR-106a	12612	20247	7824	8532	2.009	AAAAGUGCUUACAGUGCAGGUAG
miR-7	67	35	20	23	2.379	UGGAAGACUAGUGAUUUUGUUGU
miR-182	3253	1700	652	771	3.480	UUUGGCAAUGGUAGAACUCACACU
miR-183	150	104	44	41	2.980	UAUGGCACUGGUAGAAUUCACU
miR-221	6920	6875	3409	3241	2.074	AGCUACAUUGUCUGCUGGGUUUC
miR-223	123	129	23	44	3.759	UGUCAGUUUGUCAAAUACCCCA
miR-224	115	246	53	29	4.365	CAAGUCACUAGUGGUUCCGUU
miR-224*	251	420	169	149	2.116	AAAAUGGUGCCCUAGUGACUACA
miR-125b-2*	55	62	26	22	2.417	UCACAAGUCAGGCUCUUGGGAC
miR-188	76	166	63	41	2.309	CAUCCCUUGCAUGGUGGAGGG
miR-200c	83	79	43	33	2.118	CGUCUUACCCAGCAGUGUUUGG
miR-135b	20	46	13	13	2.532	AUGUAGGGCUAAAAGCCAUGGG
miR-424	197	268	40	49	5.261	CAAAACGUGAGGCGCUGCUAU
miR-18b	174	336	72	83	3.294	UAAGGUGCAUCUAGUGCAGUUAG
miR-431	40	55	25	16	2.302	UGUCUUGCAGGCCGUCAUGCA
miR-452	59	125	52	31	2.228	AACUGUUUGCAGAGGAAACUGA
miR-181d	154	187	88	52	2.434	AACAUUCAUUGUUGUCGGUGGGU
miR-503	257	404	21	56	8.595	UAGCAGCGGGAACAGUUCUGCAG
miR-509-3p	39	44	15	12	3.035	UGAUUGGUACGUCUGUGGGUAG
miR-552	157	147	86	60	2.077	AACAGGUGACUGGUUAGACAA
miR-584	53	109	46	27	2.219	UUAUGGUUUGCCUGGGACUGAG
miR-550*	153	92	45	34	3.118	UGUCUUACUCCCUCAGGCACAU
miR-622	97	70	37	22	2.814	ACAGUCUGCUGAGGUUGGAGC
miR-421	467	513	148	119	3.666	AUCAACAGACAUUAAUUGGGCGC
miR-542	39	57	21	25	2.076	UCGGGGAUCAUCAUGUCACGAGA
miR-1244	43	74	34	19	2.206	AAGUAGUUGGUUUGUAUGAGAUGGUU
miR-3178	4584	1515	1072	1792	2.130	GGGGCGCGGCCGGAUCG
miR-3200	38	62	24	21	2.226	CACCUUGCGCUACUCAGGUCUG
下调
miR-139-5p	75	95	288	331	0.276	UCUACAGUGCACGUGUCUCCAG
miR-139-3p	26	18	43	82	0.352	GGAGACGCGGCCCUGUUGGAGU
miR-215	393	323	565	955	0.471	AUGACCUAUGAAUUGACAGAC
miR-200b	1279	1071	2271	2827	0.461	UAAUACUGCCUGGUAAUGAUGA
miR-133a	28	36	61	99	0.405	UUUGGUCCCCUUCAACCAGCUG
miR-150	1892	820	2894	3659	0.414	UCUCCCAACCCUUGUACCAGUG
miR-195	1669	2106	5301	4240	0.396	UAGCAGCACAGAAAUAUUGGC
miR-194*	565	390	573	1364	0.493	CCAGUGGGGCUGCUGUUAUCUG
miR-375	1490	1099	6325	6195	0.207	UUUGUUCGUUCGGCUCGCGUGA
miR-378*	207	171	613	546	0.326	CUCCUGACUCCAGGUCCUGUGU
miR-378	4194	3168	9494	10281	0.372	ACUGGACUUGGAGUCAGAAGG
miR-342	124	96	264	199	0.477	AGGGGUGCUAUCUGUGAUUGA
miR-133b	22	25	42	59	0.469	UUUGGUCCCCUUCAACCAGCUA
miR-422a	583	573	2067	1291	0.344	ACUGGACUUAGGGUCAGAAGGC
miR-574	3042	2515	4567	7373	0.465	CACGCUCAUGCACACACCCACA
miR-650	91	121	179	306	0.438	AGGAGGCAGCGCUCUCAGGAC
miR-378c	1855	1442	4579	3743	0.396	ACUGGACUUGGAGUCAGAAGAGUGG
miR-4281	642	700	996	1766	0.486	GGGUCCCGGGGAGGGGGG

表3 miR-375和miR-31预测可能的靶基因

靶基因	基因全称	文献数目	报道时间	预测结合位点
miR-375
ZFHX4	ZINC FINGER HOMEODOMAIN 4	1	2010[7]	表4
miR-31
BACH2	BTB AND CNC HOMOLOGY 1, BASIC LEUCINE ZIPPER TRANSCRIPTION FACTOR 2	1	2006[8]	表5
FZD3	FRIZZLED HOMOLOG 3	1	2010[9]	表6
PEX5	PEROXISOMAL BIOGENESIS FACTOR 5	1	1999[10]	表7
PPP6C	PROTEIN PHOSPHATASE 6, CATALYTIC SUBUNIT	1	2004[11]	表8
RGS4	REGULATOR OF G-PROTEIN SIGNALLING 4	1	2008[12]	表9

表4 miR-375与ZFHX4间潜在的结合位点

Has-miR-375/ZFHX4 Alignment	Scores
3'agugcgcUCGGCUU--GCUUGUUu 5'hsa-miR-375	mirSVR score: -0.1512
1065: 5'cuguuuaAGUACAAGUUGAACAAa 3'ZFHX4	PhastCons score: 0.7441

表5 miR-31与BACH2间潜在的结合位点

Has-miR-31/BACH2 Alignment	Scores
3'ucgaUACGGUCGUAGAACGGa 5'hsa-miR-31	mirSVR score: -0.0003
191: 5'aaugGUGCUA-CCCCUUGCCc 3'BACH2	PhastCons score: 0.6757

表6 miR-31与FZD3间潜在的结合位点

Has-miR-31/FZD3 Alignment	Scores
3'ucgAUACGGUCGUAGAACGGa 5'hsa-miR-31	mirSVR score: -0.9748
927: 5'cagUGAGAUGUGAUCUUGCCa 3'FZD3	PhastCons score: 0.7053

表7 miR-31与PEX5间潜在的结合位点

Has-miR-31/PEX5 Alignment	Scores
3'ucgauACGG-UCGUAGAACGGa 5'hsa-miR-31	mirSVR score: -0.4073
1075: 5'augacUGGUAAGCCUCUUGCCu 3'PPP6C	PhastCons score: 0.6264

表8 miR-31与PPP6C间潜在的结合位点

Has-miR-31/PPP6C Alignment	Scores
3'ucgaUACGGUCGUAGAACGGa 5'hsa-miR-31	mirSVR score: -0.6540
101: 5'ugaaAUGCU-GCCUCUUGCCu 3'PPP6C	PhastCons score: 0.7319

表9 miR-31与RGS4间潜在的结合位点

Has-miR-31/RGS4 Alignment	Scores
3'ucGAUACGGUCGUAGAACGGa 5'hsa-miR-31	mirSVR score: -0.6450
1906: 5'aaCUUUAUUGG--UCUUGCCc 3'RGS4	PhastCons score: 0.6315