应用基因芯片对人肝纤维化相关基因的筛查

表1 部分表达上调的基因

Refseq No.	Symbol	Description	Control	Fibrosis	F/C
NM_004995	MMP-14	Matrix metallopeptidase 14	204	4793	23.46
NM_183048	PRKCBP1	Protein kinase C BP1	2901	20 215	6.97
NM_002624	PFDN5	Prefoldin 5	1629	11 312	6.94
NM_002421	MMP1	Matrix metallopeptidase 1	101	632	6.23
NM_002754	MAPK13	Mitogen-activated PK 13	62	261	4.22
NM_005356	LCK	Lymphocyte-specific PTK	1442	5135	3.56
NM_005234	NR2F6	Nuclear receptor subfamily 2F6	264	769	2.91
NM_016155	MMP-17	Matrix metallopeptidase 17	1435	2821	1.97
NM_007312	HYAL1	Hyaluronoglucosaminidase 1	1372	2658	1.94
NM_002165	ID1	Inhibitor of DNA binding 1	4082	7816	1.91
NM_002343	LTF	Lactotransferrin	2552	4541	1.78
NM_002629	PGAM1	Phosphoglycerate mutase 1	5175	9033	1.75
NM_002502	NFKB2	Nuclear factor of kappa light	4761	7481	1.57
NM_000545	TCF1	Transcription factor 1, hepatic	6530	10 047	1.54
NM_000224	KRT18	Keratin 18	2807	4172	1.49

表2 部分表达下调的基因

Refseq No.	Symbol	Description	Control	Fibrosis	F/C
NM_001812	CENPC1	Centromere protein C 1	5159	508	0.1
NM_006568	CGRRF1	Cell growth regulator	5634	808	0.14
NM_005211	CSF1R	Colony stimulating factor 1R	3147	533	0.17
NM_000964	RARA	Retinoic acid receptor, alpha	6005	1379	0.23
NM_005845	ABCC4	ATP-binding cassetteC4	7384	1701	0.23
NM_001806	CEBPB	(C/EBP), beta	3154	822	0.26
NM_001789	CDC25A	Cell division cycle 25A	12 598	3945	0.31
NM_000051	ATM	Ataxia telangiectasia mutated	13 336	4333	0.32
NM_145904	HMGA1	High mobility AT-hook 1	3157	1090	0.35
NM_000965	RARB	Retinoic acid receptor, beta	6240	2162	0.35
NM_004358	CDC25B	Cell division cycle 25B	13 381	4759	0.36
NM_004071	CLK1	CDC-like kinase 1	2476	962	0.39
NM_020990	CKMT1B	Creatine kinase 1B	3113	1240	0.4
NM_001686	ATP5B	ATP synthase	11 049	4403	0.4

表3 C/EBPβ和MMP-14基因mRNA测定结果 (mean±SD, n = 6)

分组	C/EBPβ	MMP-14
正常肝	59.13±8.21	21.65±4.37
肝纤维化	22.02±4.82b	257.33±26.58b

^bP<0.01 vs 正常组.