亚硝胺诱导食管癌变早期基因差异表达与表观遗传修饰的关系

表1 部分基因差异表达的时段特点

基因	GenBank ID	Signal Log Ratio
		4 wk	8 wk	20 wk
Akt3	NM_011785	-	-	-
Met	NM_008591	-	-	-
Otc	NM_008769	-	-	-
Alb1	NM_009654	9.77	-	-
Mup1	NM_031188	21.88	-	-
Apoh	NM_013475	6.59	-	-
Rras	NM_009101	-	2.72	-
Ski	NM_011385	-	4.86	-
Frat2	NM_177603	-	2.59	-
Ets1	NM_011808	-	-	7.54
Fgr	A130081E08	-	-	2.55
Kit	NM_021099	-	-	2.71
Mafa	NM_194350	-	-	2.8
Mos	NM_020021	-	-	4.62
Mybl2	NM_008652	-	-	2.39
Rel	NM_009044	-	-	5.8
Bcl2	6030439K11	-	-	12.46
Braf	AK036798	-	-	5.46
Cdk2	NM_183417	-	-	3.00
Dntt	NM_009345	-	-	29.08
Plg	NM_008877	-	-	6.57
Sod1	AK080908	-	-	109.38
Crkl	NM_007764	-	2.31	3.80
Fos	NM_010234	-	15.84	10.52
Maff	NM_010755	-	32.53	22.96
Mafk	NM_010757	-	14.24	11.20
Mafg	NM_010756	-	2.17	3.11
Myb	NM_033597	-	9.42	18.64
Araf	1200013E08	-	8.40	14.80
Kras2	NM_021284	-	13.77	55.81
Mras	NM_008624	-	16.00	16.46
Nras	NM_010937	-	9.83	14.42
Src	NM_009271	-	2.40	3.46
Yes	NM_009535	-	2.70	10.42
Abl1	NM_009594	-	19.13	12.46
Crk	NM_133656	2.10	38.40	57.81
Junb	NM_008416	3.56	16.49	83.45
Jun	NM_010591	6.27	169.95	94.38
Myc	NM_010849	2.34	55.93	37.98
Raf1	NM_029780	2.36	85.86	74.91

表2 部分差异表达显著的基因

基因	GenBank ID	Signal Log Ratio
		4 wk	8 wk	20 wk
Rab5a	NM_025887	2.59	56.35	102.30
Rab11a	NM_017382	2.32	55.23	72.80
Raf1	NM_029780	2.36	85.86	74.91
Hras1	NM_008284	4.53	89.70	87.69
Rab6	NM_024287	4.25	72.04	101.41
Rab14	NM_026697	11.70	346.95	248.19
Rab7	NM_009005	4.56	193.14	258.92
Mafb	NM_010658	0.00	0.00	990.00
Rab3d	6720486C03	0.00	20.76	14.86
Rab2	NM_021518	0.00	40.28	58.38
Vav3	NM_146139	0.00	28.00	42.46
Rab34	6430710G15	0.00	30.48	32.74
Rab10	NM_016676	0.00	31.33	40.31
Rab8a	NM_023126	0.00	36.00	39.76
Rab11b	NM_008997	0.00	59.47	33.87
Akt1	NM_009652	0.00	65.78	45.00
Rala	NM_019491	2.18	70.56	100.05
Hras1	NM_008284	4.53	89.70	87.69
Calm4	NM_020036	3.49	548.92	784.20
Calm2	NM_007589	11.36	472.14	847.66
Cycs	NM_007808	17.10	169.20	336.39
Mt4	NM_008631	7.69	87.61	77.89
Snx1	NM_019727	4.48	76.10	139.85
Ccnh	1810059M17	6.32	67.67	102.66
Psmc6	NM_025959	22.00	274.50	1134.35
Eif2s2	2700038J22	24.80	393.75	1761.23
Creg	1600000E17	22.00	1457.10	1430.31
Pts	0610012K15	0.00	392.40	558.00
Fth	NM_010239	14.24	807.34	319.01
Cab39	NM_133781	2.89	245.9 0	115.46

表3 部分抑癌基因的差异表达

基因	GenBank ID	Signal Log Ratio
		4 wk	8 wk	20 wk
Brca1	NM_009764	0.00	0.00	3.31
Brca2	NM_009765	0.00	6.64	15.92
Pten	NM_008960	0.00	36.38	38.08
Apc	NM_007462	0.00	0.00	3.05
Ptch1	NM_008957	0.00	0.00	2.85
Dcc	NM_007831	0.00	0.00	8.11

表4 甲基化酶基因的差异表达

基因	GenBank ID	Signal Log Ratio
		4 wk	8 wk	20 wk
Dnmt1	NM_010066	0.00	5.65	6.80
Dnmt2	NM_010067	0.00	2.11	4.15
Dnmt3a	G431002P10	0.00	0.00	0.00
Dnmt3b	NM_010068	0.00	0.00	0.00

表5 去甲基酶基因的差异表达

基因	GenBank ID	Signal Log Ratio
		4 wk	8 wk	20 wk
Mbd2	NM_010773	0.00	2.29	4.00
Gadd45a	NM_007836	2.91	56.70	65.47

表6 乙酰化修饰相关基因的差异表达

基因	GenBank ID	Signal Log Ratio
		4 wk	8 wk	20 wk
Hdac2	NM_008229	0.00	0.00	15.23
Hdac10	NM_199198	0.00	20.14	25.79
Hdac7a	NM_019572	0.00	0.00	3.76
Hdac5	NM_010412	0.00	40.74	21.21
Hdac3	NM_010411	0.00	5.82	6.65
Hdac1	NM_008228	0.00	4.32	5.17
Hdac11	NM_144919	0.00	0.00	0.00
Hdac6	NM_010413	0.00	0.00	2.58