基础研究
Copyright ©The Author(s) 2007.
世界华人消化杂志. 2007-03-18; 15(8): 831-837
在线出版 2007-03-18. doi: 10.11569/wcjd.v15.i8.831
表1 RT-PCR引物和产物
基因基因库序列号位点引物序列产物大小(bp)
β-actinNM_031144F5'-AGCCATGTACGTAGCCATCC-3'228
R5'-CTCTCAGCTGTGGTGGTGAA-3'
ICAM-1NM_012967F5'-GGGGAAAGATCATACGGGTT-3'241
R5'-TTTAGGCATGGTGGTTGACA-3'
TNF-αNM_012675F5'-CTCTTCAAGGGACAAGGCTG-3'185
R5'-TGGAAGACTCCTCCCAGGTA-3'
表2 血清AST的变化(mean±SD, nkat/L)
分组1 h3 h6 h24 h
Sham1723.7±235.01797.0±238.41882.0±193.41743.7±268.4
IR5447.8±641.8b12734.2±876.8b18537.0±2567.2b6879.7±388.4b
IP4849.3±368.4bc8880.1±691.8bc14771.3±955.2bc4567.6±275.1bc
HHI-I2858.9±466.8bce6441.3±656.8bce10010.3±851.8bce2887.2±270.1bce
表3 血清ALT的变化(mean±SD, nkat/L)
分组1 h3 h6 h24 h
Sham1306.9±226.51286.3±117.21257.9±121.41217.9±123.4
IR3569.0±383.4b10163.7±713.5b15116.4±1008.5b4047.1±283.4b
IP3042.3±216.7bc6803.0±548.4bc12255.1±876.8bc2840.6±248.4bc
HHI-I2578.8±328.4bce5401.1±361.7bc8768.4±698.5bce2378.8±303.4bce
表4 血清LDH的变化(mean±SD, nkat/L)
分组1 h3 h6 h24 h
Sham7911.6±508.48095.0±386.77969.9±505.17921.6±595.1
IR19920.7±1800.4b54444.2±3535.7b80632.8±4517.6b14322.9±781.8b
IP17503.5±1070.2bc34340.2±2655.5bc42375.1±2633.9bc12164.1±735.1bc
HHI-I15669.8±1603.7bce23054.6±2185.4bce30856.2±2567.2bce10550.4±710.1bce
表5 不同组肝组织MDA含量的变化(mean±SD, nmol/g)
分组MDA
SOD
1 h3 h6 h24 h1 h3 h6 h24 h
Sham7.32±0.537.58±0.627.50±0.607.47±0.73182.50±13.10181.70±10.20183.70±10.20185.5±7.89
I/R13.80±1.19b20.72±1.34b28.70±2.29b16.07±1.738b144.10±8.03b134.50±7.45b110.80±9.82b129.9±4.98b
IP11.75±1.22bc17.48±1.71bc21.66±1.84bc12.77±1.45bc154.60±8.70b140.90±6.35b124.70±9.32bc141.6±7.74bc
HHI-I10.45±1.31bc14.49±1.89bce17.35±1.39bce11.58±2.32bc169.50±5.60ce156.20±9.88bce136.00±12.50bce149.8±8.98bc
表6 不同组肝组织TNF-α和ICAM-1的表达
分组TNF-α/β-actinICAM-1/β-actin
Sham0.32±0.040.21±0.04
I/R1.34±0.10b1.14±0.15b
IP0.78±0.08bd0.69±0.11bd
HHI-I0.54±0.06bdf0.43±0.03bdf

引文著录: 毕旭东, 郭伟, 赵晶, 赵连根, 付晓光. 活血化瘀注射液I号预处理与缺血预处理改善肝缺血再灌注损伤. 世界华人消化杂志 2007; 15(8): 831-837