人大肠癌转移相关基因片段的筛选和鉴定

表1 Differential Screening 杂交结果判断标准

探针类型				杂交结果分析
A	R	1-C	2-C
+	-	+	-	阳性差异表达结果
+	-	-	-	阳性差异表达基因(强烈提示低丰度表达差异基因)
+	-	+	+	多为假阳性结果，但是也可能模板与不同长度地探针杂交造成结果难以判断，多为多基因家族成分
++	+	+	±	绝少情况为差异表达基因
+	+	+	+	非阳性差异表达基因
-	-	-	-	非阳性差异表达基因

表2 11个差异片段与人已知基因的同源性

Clone(bp)	Known gene description	Acc1ession	Identities	Expression
A80(400)	Heat Shock 10kD	NM_002157	200/209 (95%) in sw620	Protein1 (HSP10)
A98(138)	Cytochrome C	NM_173705	75/75(100%) in sw620	OxidaseII (COXII)
A108(181)	Mitotic Control Protein	NM_014953	130/131(99%) in sw620	dis3 homolog(DIS3)
A164(300)	skeletal muscle actin	NM_001100	167/168(99%) in sw620	alpha 1 (ACTA1)
A184(301)	Serum amyloid A(SAA)	NM_030754	276/276 (100%) in sw620
R159(91)	egl nine homolog 2	NM_080732	90/91(98%) in sw480	(EGLN2)
R57(450)	Eukaryotic Translation	NM_001416	448/450(99%) in sw480	Initiation Factor 4A(ETIF4A)
R178(298)	Glutathione S-transferase	AF043105	260/264(98%) in sw480	mu 3 (GSTM3)
R211(155)	Similar to Cytochrome c	BC013930	153/155(98%) in sw480	Oxidase III(COXIII)
R32(214)	Haplotype As9y	AY195792	213/213(100%) in sw480	mitochondrion