原发性肝癌DNA修复酶hOGG1, hMTH1 基因表达与DNA氧化损伤的修复

表1 HCC及癌旁组织8-OHdG含量, hMTH1, hOGG1, Mn-SOD和GSH-Px的表达M(n = 23)

组织	8-OHdG含量(nmol/g DNA)	hMTH 1 mRNA(hMTH1/β-actin×100)	hOGG 1 mRNA(hOGG 1/β-actin×100)	Mn-SOD mRNA(Mn-SOD/β-actin)	GSH-Px mRNA(GSH-Px/β-actin)
癌旁	133 (27-387)	0.256 (0.062-1.917)	1.256 (0.392-5.782)	0.190 (0.114-1.114)	1.652 (0.615-9.082)
HCC	56 (16-361)b	0.476 (0.083-4.861)a	1.196 (0.370-6.687)	0.186 (0.010-1.257)	0.980 (0.211-13.931)

^aP<0.05,

^bP<0.01 vs 癌旁.

表2 引物序列

hOGG1	5'-ACA CTG GAG TGG TGT ACT AGCG-3'	Genbank: AB000410
	5'-GCG ATG TTG TTG TTG GAG G-3'
hMTH1	5'-AGC CTC AGC GAG TTC TCC TG-3'	Genbank: AK026631
	5'-GAT CTG GCC CAC CTT GTG C-3'
Mn-SOD	5'-GAG ATG TTA CAG CCC AGA TAG C-3'	Genbank: Y00472
	5'-AAT CCC CAG CAG TGG AAT AAG G-3'
GSH-Px	5'-TGC TCG GCT TCC CGT GCA A-3'	Genbank: M21304
	5'-ACC GTT CAC CTC GCA CTT CT-3'
β-Actin	5'-CGG GAA ATC GTG CGT GAC AT-3'	Genbank: NM001101
	5'-GAA CTT TGG GGG ATG CTC GC-3'