shRNA干预IGF-ⅠR活化对肝癌细胞增殖的抑制作用

图1 IGF-ⅠR-shRNA转染肝癌细胞荧光学显微镜观察. A, B: Bel-7404细胞; C, D: PLC/PRF/5细胞. A, C: 荧光显微镜图; B, D: 同一视野普通光镜图. IGF-ⅠR: 胰岛素样生长因子-Ⅰ型受体; shRNA: 短发夹型RNA.

图2 shRNA转染抑制Bel-7404及PLC/PRF/5细胞IGF-ⅠR表达(n = 3). A: Bel-7404细胞IGF-ⅠR mRNA抑制率; B: PLC/PRF/5细胞IGF-ⅠR mRNA抑制率; C, E: Western blot分析Bel-7404细胞IGF-ⅠR表达; D, F: Western blot分析PLC/PRF/5细胞IGF-ⅠR表达. 1: Control组; 2: Negative-shRNA; 3: IGF-ⅠR-shRNA1; 4: IGF-ⅠR-shRNA2; 5: IGF-ⅠR-shRNA3; 6: IGF-ⅠR-shRNA4. IGF-ⅠR: 胰岛素样生长因子-Ⅰ型受体; shRNA: 短发夹型RNA.