低表达Smad4对肝癌细胞SMMC-7721上皮间质转化的影响

图1 Western blot法检测SMMC-7721细胞敲低Smad4基因后相应蛋白的表达. ^aP<0.05 vs CON组和RNAi-NC组; 1: CON; 2: RNAi-NC; 3: RNAi-Smad4-12; 4: RNAi-Smad4-2.

图2 各Western blot法检测TGF-β1刺激前后对Smad4敲低表达组β-catenin总蛋白表达的影响. ^aP<0.05 vs 未受刺激的CON组和RNAi-NC组; ^cP<0.05 vs TGF-β1孵育48 h后的CON组和RNAi-NC组; 1: CON; 2: RNAi-NC; 3: RNAi-Smad4-12; 4: RNAi-Smad4-2; A,B: β-catenin; C, D: β-actin.

图3 Western blot法检测TGF-β1刺激前后Smad4敲低表达组Vimentin蛋白的表达. ^aP<0.05 vs 未受刺激的CON组和RNAi-NC组; ^cP<0.05 vs TGF-β1孵育48 h后的CON组和RNAi-NC组; 1: CON; 2: RNAi-NC; 3: RNAi-Smad4-12; 4: RNAi-Smad4-2; A, B: Vimentin; C, D: β-actin.

图4 RT-PCR法检测Smad4低表达后β-catenin和Vimentin mRNA的表达. ^aP<0.05 vs CON组和RNAi-NC组; A: β-catenin; B: Vimentin; 1: CON; 2: RNAi-NC; 3: RNAi-Smad4-12; 4: RNAi-Smad4-2.

图5 Smad4细胞荧光的表达. A, B, C, D: Smad4; E, F, G, H: DAPI核染; A, E: CON; B, F: RNAi-NC; C, G: RNAi-Smad4-12; D, H: RNAi-Smad4-2.

图6 Smad4敲低表达后β-catenin和Vimentin荧光表达. A-H: β-catenin; I-P: Vimentin; A, B, C, D, I, J, K, L: β-catenin/Vimentin; E, F, G, H, M, N, O, P: DAPI核染; A, E, I, M: CON; B, F, J, N: RNAi-NC; C, G, K, O: RNAi-Smad4-12; D, H, L, P: RNAi-Smad4-2.