研究快报
Copyright ©The Author(s) 2010.
世界华人消化杂志. 2010-03-18; 18(8): 819-824
在线出版 2010-03-18. doi: 10.11569/wcjd.v18.i8.819
图1
Open in New Tab Full Size Figure Download Figure
图1 不同培养对SMMC-7721细胞存活率的影响(TUNEL染色×400). A: 悬浮培养; B: 贴壁培养.
图2
Open in New Tab Full Size Figure Download Figure
图2 不同培养对SMMC-7721细胞存活率的影响(Hoechst33258染色×400). A: 悬浮培养; B: 贴壁培养.
图3
Open in New Tab Full Size Figure Download Figure
图3 TUNEL染色阳性率分析.
图4
Open in New Tab Full Size Figure Download Figure
图4 HGF对失巢凋亡细胞生存率的影响. ASF: 无血清培养基悬浮培养SMMC-7721; HGF: HGF处理后SMMC-7721; HGF+LY294002: LY294002预处理后加入HGF处理SMMC-7721.
图5
Open in New Tab Full Size Figure Download Figure
图5 HGF对失巢凋亡细胞增殖能力的影响. ASF: 无血清培养基悬浮培养SMMC-7721; HGF: HGF处理后SMMC-7721; HGF+LY294002: LY294002预处理后加入HGF处理SMMC-7721.
图6
Open in New Tab Full Size Figure Download Figure
图6 流式细胞仪检测各组细胞的凋亡率. A: 贴壁培养SMMC-7721; B: 悬浮培养SMMC-7721; C: HGF处理SMMC-7721; D: LY294002预处理后加HGF处理SMMC-7721.
图7
Open in New Tab Full Size Figure Download Figure
图7 HGF对细胞失巢凋亡率的影响(Hoechst33258染色). A: 贴壁培养SMMC-7721; B: 悬浮培养SMMC-7721; C: HGF处理SMMC-7721; D: LY294002预处理后加HGF处理SMMC-7721.
图8
Open in New Tab Full Size Figure Download Figure
图8 Western blot检测HGF对Akt和FAK的影响. A: 各组Akt与p-Akt的表达结果; B: 各组FAK和p-FAK的表达结果.
引文著录: 谷艳娇, 奥瑞芳, 苏荣健. 肝细胞生长因子对肝癌细胞系SMMC-7721失巢凋亡的影响. 世界华人消化杂志 2010; 18(8): 819-824