CD/5-FC自杀基因体系对肝、胆、胰肿瘤细胞的杀伤作用及机制

图1 三细胞系生长曲线. BEL-7402和BXPC-3生长速度均明显较QBC细胞快, 生长旺盛, 而BEL-7402和BXPC-3在高峰期几乎一致, 生长曲线也有交叉重叠, 差异不显著.

图2 细胞的瞬时转染率. A, D: S7402; B, E: BXPC-3; C, F: QBC, 0: 阳离子脂质体介导的转染; 1: 裸质粒的转染.

图3 细胞RNA甲醛变性电泳结果. 1: BXPC3-PCDNA3.1; 2: QBC-PCDNA3.1; 3: BEL7402-PCDNA3.1; 4: BXPC3-PCDNA3.1 -CD; 5: QBC-PCDNA3.1- CD; 6: BEL7402-PCDNA 3.1-CD.

图4 CD基因表达的RT-PCR结果. 1: Marker条带; 2: GAPDH-BXPC3-PCDNA3; 3: GAPDH-QBC-PCDNA3; 4: GAPDH-BEL7402-PCDNA3; 5: GAPDH-BXPC3 -CD; 6: GAPDH-QBC-CD; 7: GAPDH-BEL7402-CD; 8: BXPC3-PCDNA3; 9: QBC-PCDNA3; 10: BEL7402-PCDNA3; 11: BXPC3-CD; 12: QBC-CD; 13: BEL7402-CD.

图5 CD/5-FC的生长抑制效果. A: BEL-7402; B: QBC; C: BXPC3.

图6 BEL-7402、QBC、BXPC-3的凋亡. A: BEL-7402; B: QBC; C: BXPC-3.

图7 细胞凋亡. A: BEL-7402; B: QBC; C: BXPC-3.