人可溶性肿瘤坏死因子受体1基因原核表达及活性鉴定

图1 pMAL-c2x-sTNFR1克隆的酶切鉴定. 1: DNA标准分子量(λ-EcoT14); 2: 酶切后; 3: 酶切前.

图2 pMAL-c2x-sTNFR1克隆的测序鉴定.

图3 sTNFR1重组体体外表达结果鉴定. 1: 蛋白质Marker; 2: pMAL-c2x/JM109诱导后; 3: pMAL-c2x/JM109诱导前; 4: pMAL-c2x-sTNFR1/JM109诱导后; 5: pMAL-c2x-sTNFR1/JM109诱导前; 6: JM109(空菌).

图4 MBP-sTNFR1重组蛋白纯化后. 1: 蛋白质Marker; 2-5: 纯化后的sTNFR1-MBP重组蛋白; 6: pMAL-c2x-sTNFR1/JM109诱导后; 7: JM109(空菌).

图5 Western blot鉴定重组蛋白. 1: JM109(空菌); 2: pMAL-c2x/JM109诱导后; 3: pMAL-c2x-sTNFR1/JM109诱导后.

图6 sTNFR1-MBP对TNF-α细胞毒效应的抑制作用.