幽门螺杆菌融合蛋白HspA-UreB的表达和免疫学活性

图1 MEL-HP27 hspA和ureB基因的PCR扩增. 1: 100 bp DNA ladder marker; 2: PCR product of hspA gene; 3: 1 kb DNA ladder marker; 4: PCR product of ureB gene.

图2 重组质粒pET-HU27酶切鉴定图谱. 1: 1 kb DNA ladder marker; 2: pET-HU27 digested by EcoRI; 3: pET-HU27 digested by EcoRI、XhoI; 4: product of hspA-ureB fusion gene from pET-HU27.

图3 HspA-UreB融合蛋白的SDS-PAGE分析. 1: protein marker; 2-5: BL21 with recombinant plasmid pET-HU27 induced by IPTG for 4、3、2 and 1 h; 6: BL21 with recombinant plasmid pET-HU27 uninduced for 4 h; 7: BL21 with pET30a induced for 4 h.

图4 诱导3 h融合蛋白含量分析.

图5 亲和层析纯化融合蛋白结果. 1: protein marker; 2-4: purified fusion protein.

图6 融合蛋白的Western blot分析. 1: unpurified fusion protein; 2: purified fusion protein.