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©The Author(s) 2004.
图2 重组体pGEM-MCP-1和pGEM-Groα的双酶切鉴定.
M: λ-Hind Ⅲ digest; 1: pGEM-MCP-1/BamHⅠ, EcoRⅠ; 2: pGEM- Groα/BamHⅠ, Eco RⅠ.
图3 重组体pcDNA3.
1(+)-MCP-1和pcDNA3.1(+)-Groα的双酶切鉴定及PCR鉴定. M1: λ-Hind Ⅲ digest; 1: pcDNA3.1(+); 2: pcDNA3.1(+)/BamHⅠ, EcoRⅠ; 3: pcDNA3.1(+)-MCP-1; 4: pcDNA3.1(+)-MCP-1/BamHⅠ, EcoRⅠ; 5: pcDnNA3.1(+)-Groα; 6: pcDNA3.1(+)-Groα/BamHⅠ, EcoRⅠ; 7: MCP-1 target gene; 8: Groαtarget gene; M2: marker DL-2000.
引文著录: 任玥欣, 宋于刚, 陈学清, 智发朝, 钟世顺, 南清振, 武金宝, 崔忠林. pcDNA3.1(+)-MCP-1和pcDNA3.1(+)-Gro α真核表达质粒的构建及鉴定. 世界华人消化杂志 2004; 12(11): 2623-2626