原代猪肝细胞PERV检测及其意义

图1 原代猪肝细胞前病毒PCR检测. 图A 1: 猪肝细胞; 2: 兔肝细胞; 3: Marker. B 1: 正常人肝组织; 2: Marker; 3: HCV阳性肝组织; 4: 猪肝细胞PCR. C 1: Marker; 2: 阴性对照; 3: 猪肝细胞.

图2 中国实验小型猪血清PERV检测. 1: 正常成人; 2: HBV阳性; 3: HCV阳性; 4: 兔; 5: 猪套式PCR产物; 6: HCV阳性; 7: 猪未RT的PCR; 8: 猪RT-PCR; 9: Marker.

图3 普通培养上清PERV检测. 1: day 1未RT的PCR; 2: day 1 RT-PCR; 3: day 3 RT-PCR; 4: day 3 套式PCR; 5: Marker; 6: day 5 RT-PCR; 7: day 7 RT-PCR; 8: day 9 RT-PCR; 9: day 9未RT的PCR.