Gene transfer and expression of enhanced green fluorescent protein in variant HT-29c cells

Table 1 FACS analysis of EGFP expression in cell lines

Cell lines	Fluorescence intensity
HT-29c	2.5 ± 0.9a
HT-29cEGFP pool	64.6 ± 7.4b
HT-29cEGFPclone#1	206.5 ± 39.9c
HT-29cEGFPclone#7	203.4 ± 46.4d

The fluorescence intensity refers to fluorescence of 10000 cells according to standard software supplied by the FACS cytometer manufacturer. a: P < 0.001 vs b, c or d, respectively. b: P < 0.001 vs cord, c: P = 0.818 vs d.

Table 2 EGFP expression in different passages of transduced HT-29c cells by FACS

Clone #1	Fluorescence intensity
Passage 5	217.8
Passage 17	198.1
Passage 31	193.4
Passage 40	212.1
Passage 50	208.8

Table 3 Doubling times of parental HT-29 cells and EGFP transduced HT-29 cells

Cell line	Doubling time (h)a
HT-29	25.3 ± 5.5
HT-29c	26.0 ± 3.3
HT-29cEGFPclone #1	26.3 ± 4.7
HT-29cEGFPclone #7	27.7 ± 5.3

^a: F test F = 0.62, P > 0.05.

Table 4 Phenotypical comparison of HT-29cells, HT-29c cells and EGFP transduced cells

mAb	Percentage of positive cells (%)
	HT-29	T-29c	HT-29c EGFPclone
KL-1	100	100	100
IT-Ks20.10	100	100	100
C183	33.9	34.6	36.5
CA19-9	48.0	49.3	47.2
MiB-1	96.7	97.8	97.2
Do-7	96.8	97.1	98.3