Copyright
©The Author(s) 2003.
World J Gastroenterol. Sep 15, 2003; 9(9): 2083-2087
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.2083
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.2083
Table 1 FACS analysis of EGFP expression in cell lines
Cell lines | Fluorescence intensity |
HT-29c | 2.5 ± 0.9a |
HT-29cEGFP pool | 64.6 ± 7.4b |
HT-29cEGFPclone#1 | 206.5 ± 39.9c |
HT-29cEGFPclone#7 | 203.4 ± 46.4d |
Table 2 EGFP expression in different passages of transduced HT-29c cells by FACS
Clone #1 | Fluorescence intensity |
Passage 5 | 217.8 |
Passage 17 | 198.1 |
Passage 31 | 193.4 |
Passage 40 | 212.1 |
Passage 50 | 208.8 |
Table 3 Doubling times of parental HT-29 cells and EGFP transduced HT-29 cells
Cell line | Doubling time (h)a |
HT-29 | 25.3 ± 5.5 |
HT-29c | 26.0 ± 3.3 |
HT-29cEGFPclone #1 | 26.3 ± 4.7 |
HT-29cEGFPclone #7 | 27.7 ± 5.3 |
Table 4 Phenotypical comparison of HT-29cells, HT-29c cells and EGFP transduced cells
mAb | Percentage of positive cells (%) | ||
HT-29 | T-29c | HT-29c EGFPclone | |
KL-1 | 100 | 100 | 100 |
IT-Ks20.10 | 100 | 100 | 100 |
C183 | 33.9 | 34.6 | 36.5 |
CA19-9 | 48.0 | 49.3 | 47.2 |
MiB-1 | 96.7 | 97.8 | 97.2 |
Do-7 | 96.8 | 97.1 | 98.3 |
- Citation: Wang M, Boenicke L, Howard BD, Vogel I, Kalthoff H. Gene transfer and expression of enhanced green fluorescent protein in variant HT-29c cells. World J Gastroenterol 2003; 9(9): 2083-2087
- URL: https://www.wjgnet.com/1007-9327/full/v9/i9/2083.htm
- DOI: https://dx.doi.org/10.3748/wjg.v9.i9.2083