Effects of hydroxyapatite nanoparticles on proliferation and apoptosis of human hepatoma BEL-7402 cells

Table 1 Inhibitory effect of HAP nanoparticles on hepatoma cell line BEL-7402

Concentrations (mg/L)	A490nm(-x±s)	Inhibition rate (%)	t vs control
0 (control)	0.9325 ± 0.0337
12.5	0.5275 ± 0.0440bd	43.29 ± 6.11	16.68
25	0.4025 ± 0.0440b	56.90 ± 3.71	51.20
50	0.3950 ± 0.0593b	57.74 ± 5.37	37.43
75	0.4038 ± 0.0245b	56.61 ± 3.61	29.10
100	0.3913 ± 0.0323b	58.05 ± 3.05	46.20
150	0.3188 ± 0.0236bd	65.78 ± 2.83	42.30
200	0.2588 ± 0.0270bd	72.25 ± 7.75	53.79

^aP < 0.05,

^bP < 0.01 vs 0 mg/L group; ^cP < 0.05,

^dP < 0.01 vs 25 mg/L group.

Table 2 Apoptotic rates of HAP nanoparticles on hepatoma cell line BEL-7402

Concentrations (mg/L)	Apoptotic rate (%)	t vs control
0(control)	2.23 ± 0.14
50	20.35 ± 2.23b	13.32
75	25.35 ± 1.92b	22.55
100	29.34 ± 4.61b	10.50
150	44.92 ± 3.78bc	20.30
200	53.64 ± 3.49bd	24.51

^aP < 0.05,

^bP < 0.01 vs 0 mg/L group;

^cP < 0.05,

^dP < 0.01 vs 50 mg/L group.