Experimental study of anti-tumor effects of polysaccharides from Angelica sinensis

Table 1 Masses of body, tumor, thymus and spleen of mice bearing S180 10 days after being treated with AP-0, Cy and N S (^-x±s)

Group	Mice (n) (d 10/d 0)	m (body) (g)	m (tumor) (g)	m (thymus) (mg)	m (spleen) (mg)
N S	10/10	25 ± 3	2.6 ± 0.8d	69 ± 17	0.25 ± 0.08d
Cy	9/9	19 ± 3b	1.2 ± 0.4	16 ± 10b	0.06 ± 0.02
AP-0
30 mg/kg	9/10	22 ± 5	2.8 ± 1.0d	35 ± 19bd	0.22 ± 0.13d
100 mg/kg	8/10	22 ± 3	2.7 ± 0.5d	46 ± 16ad	0.20 ± 0.08d
300 mg/kg	7/10	22 ± 2	3.0 ± 1.3d	44 ± 14bd	0.34 ± 0.14d

^aP < 0.05,

^bP < 0.01 vs N S,

^dP < 0.01 vs Cy.

Table 2 Survival and body masses of mice bearing EAC after being treated with AP-0

Group	n	Survival time (days)	Life prolongation rate (%)	m (body)/(g) (-x±s)
				d 0	d 10
N S	10	14.1	0.0	19.9 ± 0.9	42.2 ± 1.9
5-FU	10	60.0	325.0	20.2 ± 0.9	24.7 ± 3.6b
AP-0
30 mg/kg	10	26.5	89.7	19.8 ± 1.6	36.5 ± 5.2b
100 mg/kg	10	18.4	30.5	19.4 ± 1.2	35.8 ± 3.0b
300 mg/kg	10	36.0	155.3a	19.3 ± 1.0	31.3 ± 3.8b

^aP < 0.05 vs N S,

^bP < 0.001 vs N S.

Table 3 Survival of mice with L1210 after being treated with AP-0 (^-x±s)

Group	n	Survival time (days)	Life prolongation rate (%)
N S	0	15.0	0.0
Cy	5	30.0	200.0
AP-0
30 mg/kg-	1	25.0	66.7a
100 mg/kg	0	23.5	56.7
300 mg/kg	0	15.0	0.0

^aP < 0.05 vs N S.

Table 4 Inhibition of Angelica polysaccharides on infiltration and migration of HHCC

Group	Infiltration		Migration
	Infiltrated cells (-x±s)	Inhibitory rate(%)	Migrated cells (-x±s)	Inhibitory rate (%)
Control	20.2 ± 18.5	0.0	23.6 ± 18.7	0.0
AP-0	8.8 ± 9.2a	56.4	29.5 ± 17.8	\
AP-1	12.4 ± 9.5	38.6	14.4 ± 4.8	39.0
AP-2	6.4 ± 2.9a	68.3	17.0 ± 7.9	28.0
AP-3	14.8 ± 14.5	26.7	16.3 ± 9.0	30.9

^aP < 0.05 vs Control.

Table 5 Influence of Angelica polysaccharides on adhesion of HHCC to Matrigel and fibronectin

Group	Matrigel		Fibronectin
	A490nm(-x±s)	Inhibitory rate (%)	A490nm(-x±s)	Inhibitory rate (%)
Control	0.131 ± 0.029	0.0	0.099 ± 0.023	0.0
AP-0	0.114 ± 0.006	12.9	0.098 ± 0.017	1.0
AP-1	0.118 ± 0.037	9.9	0.082 ± 0.004	17.2
AP-2	0.105 ± 0.018	19.8	0.073 ± 0.014	26.0
AP-3	0.098 ± 0.020	25.2	0.069 ± 0.008a	30.3

^aP < 0.05 vs Control.