Experimental study on therapeutic effect of in vivo expression of Cell I-Hep II recombinant polypeptide of fibronectin on murine H22 hepatocellular carcinoma

Table 1 Inhibitory effect of pCH510 on murine tumor inoculated with different doses of tumor cells

Inoculative doses	n	Dissecting time (d)	Tumor weight (g, -x±s)
			Control	pCH510
104	8	21	2.19 ± 0.35	0a
105	8	14	2.27 ± 0.38	0.62 ± 0.17a
106	8	10	2.32 ± 0.42	1.35 ± 0.24a
107	8	7	2.37 ± 0.33	1.88 ± 0.32b

^aP < 0.05 vs control;

^bP < 0.01 vs control.

Table 2 Inhibitory effect of pCH510 in combination with chemotherapy on murine tumor

Groups	n	Tumor weight (g, -x±s)
		d 11	d 21
A: control	8	2.12 ± 0.38
B: pCH510 (d 2 to d11)	8	0.82 ± 0.21 a
C: chemo I	8	0 a
D: chemo II	8		1.58 ± 0.31
E: MMC + pCH510 (d 2 to d 11)	8		1.49 ± 0.28
F: MMC + pCH510 (d 6 to d 20)	8		0.42 ± 0.12b

^aP < 0.01 vs control;

^bP < 0.01 vs chemo II.

Table 3 Effects of MMC on activity of macrophages

Groups	n	4 h		24 h
		Metabolism OD570, (-x±s)	NO mol/L, (-x±s)	Metabolism OD570, (-x±s)	NO mol/L, (-x±s)
Control	6	0.35 ± 0.06	29.9 ± 5.1	0.32 ± 0.04	28.8 ± 6.3
MMC	6	0.15 ± 0.03a	16.5 ± 4.3a	0.16 ± 0.04a	13.9 ± 5.4a

^aP < 0.01 vs control.

Table 4 Inhibitory effect of pCH510 + Hsp70-H22 antigen peptide complex on murine tumor

Group	n	105 H22 cells inoculation		106 H22 cells inoculation
		Dissecting time (d)	Tumor weight (g, -x±s)	Dissecting time (d)	Tumor weight (g, -x±s)
Control	8	14	3.24 ± 1.22	10	3.45 ± 1.31
pCH510 plasmid	8	21	2.38 ± 0.84	21	2.72 ± 1.28
Hsp70-H22 peptide	8	21	1.44 ± 0.45	21	2.58 ± 0.93
Hsp70-H22 peptide + pCH510	8	21	0b	21	1.76 ± 0.66a

^aP < 0.05 vs control;

^bP < 0.01 vs control.

Table 5 Inhibitory effect of chemotherapy + pCH510 + Hsp70-H22 peptide on murine tumor

Group	n	H22 cell inoculating	Dissecting time (d)	Tumor weight (g, -x±s)
Control	8	106	10	3.28 ± 1.12
MMC	8	106	15	2.82 ± 0.94
MMC + pCH510	8	106	30	1.51 ± 0.53
MMC + Hsp70-H22 peptide	8	106	30	0.62 ± 0.22b
MMC + pCH510 + Hsp70-H22 peptide	8	106	40	0a

^aP < 0.01 vs control;

^bP < 0.05 vs (MMC + pCH510).