Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228

Table 1 Counts and survival rate of suspension CCl-228 cells after CO2 or N2 treatment

Duration	Counts of suspension cells (1000/ml)		Survival rate of suspension cells
	CO2 group	N2 group	CO2 group	N2 group
0 min	4.2 ± 0.1		0.884 ± 0.011
30 min	7.5 ± 2.9	4.9 ± 2.5	0.912 ± 0.018	0.852 ± 0.022
60 min	16.7 ± 5.4ab	5.2 ± 0.7	0.914 ± 0.041	0.897 ± 0.021
120 min	23.2 ± 10.7ac	8.5 ± 1.4d	0.904 ± 0.034	0.912 ± 0.023
180 min	32.1 ± 5.5ac	9.7 ± 3.3d	0.845 ± 0.028	0.912 ± 0.032

^aP<0.01, vs the 0 min in same group;

^bP<0.05, vs N₂ group at same time;

^cP<0.01, vs N₂ group at same time;

^dP<0.05, vs 0 min in same group.