Expression of local renin and angiotensinogen mRNA in cirrhotic portal hypertensive patient

Table 1 Primer sequences

MRNA	Sequence	Size of products
Renin sense	5'-TCTCAGCCAGGACATCATCA-3'	288 bp
antisense	5'-AGTGGAAATTCCCTTCGTAA-3
Angiotensinogen		256 bp
sense	5'-TGTTGCTGCTGAGAAGATTG-3'
antisense	5'-CCGAGAAGTTGTCCTGGATG-3'

Table 2 Expression of local renin (Lr) and angiotensinogen(Lan) mRNA in the patient and control groups

	Control group (n = 12)			Patient group (n = 20)
	Liver	Splenic artery	Splenic vein	Liver	Splenic artery	Splenic vein
Lr	0.19 ± 0.11a	0.45 ± 0.17	0.39 ± 0.12	0.78 ± 0.28b	0.86 ± 0.35b	0.81 ± 0.22b
Lan	0.64 ± 0.21a	0.41 ± 0.15	0.35 ± 0.18	0.96 ± 0.25b	0.83 ± 0.18b	0.79 ± 0.23b

The data were expressed as mean ± SD. Lr and Lan mRNA in the patient group versus those in the control group,

^bP < 0.05.Within the same group, Lr and Lan mRNA in the liver versus those in splenic artery or vein respectively,

^aP < 0.05.