Esophageal Cancer
Copyright ©The Author(s) 2003.
World J Gastroenterol. Mar 15, 2003; 9(3): 417-422
Published online Mar 15, 2003. doi: 10.3748/wjg.v9.i3.417
Table 1 Clinical and pathological data of 11 cases with esophageal carcinoma
No. of in-PNameSexAgePathological diagnosisLymph metastasisClinical stage
106811Xu_F65Middle esophageal, marrow type, well-middle d.
Scc, exterior layer invasion with tumor embolus0/18II
106850Xu _F58Middle esophageal, marrow type, Poor d. Scc with
Exterior layer invasion1/8III
106839Yu _M60Middle-inferior esophageal, marrow type, well-middle d.
Scc, exterior layer invasion with tumor embolus0/26II
107001Zhou _M53Middle esophageal, ulcer type, middle d. Scc,
Exterior layer invasion with tumor embolus4/19III
107151Wang_M42Middle-up esophageal, marrow type, middle d.
Scc with exterior layer invasion0/8II
107326Gu _M51Middle- inferior esophageal, marrow type,
Middle-Poor d. Scc with exterior layer invasion2/14III
107546Zhao_F63Middle- inferior esophageal, ulcer type, well-
Middle d. Scc with shallow muscle layer invasion0/15II
107564Lu_M61Middle- inferior esophageal, marrow type,
Middle d. Scc with deep muscle layer invasion1/32III
107146Chen_F65Superior esophageal, ulcer type, well d.
Scc with exterior layer invasion0/17II
107527Xu_M49Inferior esophageal, ulcer type, middle d.
Scc with shallow muscle layer invasion1/18III
107531Guan_M56Middle esophageal, marrow type, middle d.
Scc with deep muscle layer invasion0/26II
d.: differentiated Scc: squamous cell carcinoma.
Table 2 Genes with expression difference > 3 or < 0.33 on average ratio between esophageal carcinoma and normal epithelium
Genbank-IDFcfGene nameR1R2AR
hsu67963Human lysophospholipase homolog(HU-K5)mRNA, complete cds.0.110.160.14
Hslimdp8H.sapiens mRNA for ZNF185 gene.0.150.230.19
45051660.130.260.19
d8745115Human mRNA for KIAA0262gene,complete cds0.130.310.22
hsy1540911Homo sapiens mRNA for putative glucose 6-phosphate translocase.0.280.230.25
hsu1699612Human protein tyrosine phosphatase mRNA, complete cds.0.310.260.29
hsu3025512Human phosphogluconate dehydrogenase (hPGDH) gene, complete cds.0.290.300.29
45047140.470.160.31
ab00234915Human mRNA for KIAA0351 gene, complete cds.0.140.500.32
humpai213Human placental plasminogen activator inhibitor mRNA, complete cds.0.280.350.32
hsu73824Human p97 mRNA, complete cds.0.300.340.32
hsa1322702Homo sapiens mRNA for integral type I protein p24B (p26).0.310.340.33
Humorfkg1f11Human mRNA for KIAA0058 gene,complete cds.0.310.360.33
s744523Cyclin B1{promoter region}[human,cervical carcinoma cell line, HeLa cells,Genomic, 351 nt].3.212.793.00
hspros2710H.sapiens PROS-27 mRNA.3.292.853.07
Hstrr10Human mRNA for transferrin receptor.2.703.483.09
Humprion15Homo sapiens mRNA for prion protein, complete cds.3.073.193.13
hshr21spa13H.sapiens mRNA for protein involved in DNA double-strand break repair.2.294.013.15
AB00109S1411Homo sapiens gene for H-cadherin, exon 14 and complete cds.2.953.503.23
hsranbp111H.sapiens mRNA for RanBP1.3.982.573.27
Hsxprf8Homo sapiens mRNA for putative transcription factor XPRF.3.353.383.37
humcd362111Human antigen CD36 (clone 21) mRNA, complete cds.3.283.543.41
Humsparc5Human SPARC/osteonectin mRNA, complete cds.2.873.963.42
Humhcamap35Human gene for hepatitis C-associated microtubular aggregate protein p44, exon 4.3.723.303.51
Humprofii5Human profilin II mRNA, complete cds.3.074.073.57
57297552.354.883.61
Hsrnasmg13H.sapiens mRNA for Sm protein G.2.914.573.74
Humpgc5Human chondroitin sulfate proteoglycan core protein mRNA, 3' end.3.673.913.79
hstop2a108Homo sapiens topoisomerase II alpha (TOP2A) gene,exons 34 and 35,and complete cds.4.023.763.89
hsu0955914Human Rch1 (RCH1) mRNA, complete cds.3.754.033.89
humcol1a42Human alpha-1 collagen type IV gene, exon 52.4.373.694.03
hsinta6r11Human mRNA for integrin alpha 6.3.285.434.35
d8698315Human mRNA for KIAA0230 gene, partial cds.4.714.844.78
hsu26555Human versican V2 core protein precursor splice-variant mRNA, complete cds.6.935.916.42
hspgp9513Human mRNA for protein gene product (PGP) 9.5.5.667.546.60
af05212415Homo sapiens clone 23810 osteopontin mRNA, complete cds.11.774.638.20
HumcolvaHuman alpha-2 type V collagen gene, 3' end.7.6410.178.91
humca1xia15Human alpha-1 type XI collagen (COL11A1) mRNA, complete cds.6.7512.079.41
Hscalbr11Human mRNA for 27-kDa calbindin.6.7212.179.45
hsu500788Human guanine nucleotide exchange factor p532 mRNA, complete cds.12.2817.1114.70
Hsop5Human mRNA for osteopontin.20.2320.2220.23
Fcf: function classification average ratio > 3: up regulation average ratio < 0.33: down regulation; R1: the first ratio; R2: the second ratio; AR: R1 + R2 average ratio.
Table 3 Function classification for genes showing expression differences in esophageal carcinoma and pericancerous epithelium that were > 2 or < 0.5 fold from the control tissue
Gene function classificationCarcinoma/normal
Pericancerous/normal
N> 2< 0.5N> 2< 0.5
1Proto-oncogenes and tumor suppression genes211000
2Cell signals and transducing proteins321330
3Cell cycle proteins651000
4Extra-pressure reaction proteins101000
5Cell regulatory proteins770330
6Cell apoptosis related proteins110101
7DNA synthesis, repair and recombinant proteins330000
8DNA binding, transcription and its factor642110
9Cell receptors000000
10Cell surface antigen and adhesion proteins1082440
11Ion-channel and transporters241410220
12Metabolism-related proteins211011642
13Protein synthesis-related genes1477211
14Development-related genes110220
15Other genes27189770
16New genes945000
Total135855031274
Table 4 Genes showing expression difference between the esophageal carcinoma and pericancerous epithelium
Genbank-IDFcfGene namePRTR
Hscalbr11Human mRNA for 27-kDa calbindin.2.919.45
af05212415Homo sapiens clone 23810 osteopontin mRNA, complete cds.5.348.20
Hsinta6r11Human mRNA for integrin alpha 6.2.934.35
Humprofii5Human profilin II mRNA, complete cds.2.503.57
Humhcamap35Human gene for hepatitis C-associated microtubular aggregate protein p44, exon 4.2.793.51
hum927a10Human interferon-inducible protein 9-27 mRNA, complete cds.2.432.92
Hstrr10Human mRNA for transferrin receptor.3.763.09
hsu500788Human guanine nucleotide exchange factor p532 mRNA, complete cds.18.714.7
Humtfrr10Human transferrin receptor mRNA, complete cds.3.512.81
hsu3025512Human phosphogluconate dehydrogenase(hPGDH)gene, complete cds.0.480.29
hsu056842Homo sapiens dihydrodiol dehydrogenase mRNA, complete cds.3.752.31
Hsop5Human mRNA for osteopontin.37.120.2
Hspgp9513Human mRNA for protein gene product (PGP) 9.5.19.46.60
hsu4657113Human tetratricopeptide repeat protein (tpr2) mRNA, complete cds.0.41
Humrsc3906Human mRNA for KIAA0018gene, complete cds.0.44
hcox4gn12Homo sapiens hypothetical protein (COX4AL) gene, partial cds, and cytochromec oxidase subunit IV precursor (COX4) gene, complete cds.0.47
d79997Human mRNA for KIAA0175 gene, complete cds.2.05
ab01755s1015Homo sapiens IGSF4 gene, exon 10 and complete cds.2.06
Hsnov15H.sapiens mRNA for novel gene in Xq28 region.2.09
hsifi56rHuman mRNA for 56-KDa protein induced by interferon.2.1
af05418212Homo sapiens mitochondrial processing peptidase beta-subunit mRNA, complete cds.2.11
af13181815Homo sapiens clone 25237mRNA sequence.2.19
af0353012Homo sapiens clone 23876 neuronal olfactomedin-related ER localized protein mRNA, partial cds.2.28
Humorfi14Homo sapiens (clone S240ii117/zap112) mRNA, complete cds.2.26
af03866012Homo sapiens chromosome 1p33-p34 beta-1,4-galactosyltransferase mRNA, complete cds.2.32
d8868712Homo sapiens mRNA for KM-102-derived reductase-like factor,complete cds.2.51
hsu0559812Human dihydrodiol dehydrogenase mRNA, complete cds.3.07
Humalcam10Homo sapiens CD6 ligand (ALCAM) mRNA, complete cds.3.07
hsu792992Human neuronal olfactomedin-related ER localized protein mRNA,partial cds.3.33
ab00347614Homo sapiens mRNA for gravin, complete cds. 3.65
af10075715Homo sapiens COP9 complex subunit 4 mRNA, complete cds. 5.96
Fcf: function classification; TR: esophageal carcinoma average ratio; PR: pericancerous epithelium average ratio.