Detection of type IV collagenase activity in malignant ascites

Table 1 Patient data

Characteristics	n
Number of patients	67
Male	38
Female	29
Median age (y)
Male	50.68
Female	48.60
Disease categories
Cirrhotic ascites	36
Tuberculous ascites	8
Malignant ascites	23
Ovarian cancer	8
Gastric cancer	6
Colon cancer	5
Hepatocellular cancer	2
Pancreatic cancer	1
Primary peritoneal carcinoma	1

Table 2 Qualitative analysis of type IV collagenase activity in various kinds of ascites

Ascites type	MMP-2 (Positive cases)	MMP-9 (Positive cases)
Cirrhotic ascites	0	0
Tuberculous ascites	0	0
Malignant ascites	20	18
Gastric cancer	5	5
Colon cancer	3	5
Hepatocellular cancer	2	1
Pancreatic cancer	1	0
Ovarian cancer	8	6
Primary peritoneal carcinoma	1	1

Table 3 Semi-quantitative analysis of MMP-2, MMP-9 activity in different kinds of malignant ascites

Type	Case (n)	MMP-2 activity (n)			MMP-9 activity (n)
		+/-	1+	2+	+/-	1+	2+
Gastric cancer	6	1	4	0	3	2	0
Colon cancer	5	1	2	0	4	1	0
Hepatocellular cancer	2	1	1	0	0	1	0
Pancreatic cancer	1	0	1	0	0	0	0
Ovarian cancer	8	1	5	2	3	1	2
Primary peritoneal carcinoma	1	0	0	1	0	0	1

+/-, faint band detected, < 1.0 mm in width; 1+, clear band detected, 1.0-1.5 mm in width; 2+, intense band detected, 1.5-3.0 mm in width.

Table 4 Comparison of detection of type IV collagenase and other indexes in malignant ascites

	Type IV collagenase		LDH in ascites/serum LDH (> 1: positive)	Cytologicexamination	Serum complex indexes (AFP, CEA, CA)
	MMP-2	MMP-9
Positive numbers	20	18	10	11	13
Positive rates	87.0%	78.3%	43.4%	47.8%	56.5%