Expression of exogenous rat collagenase in vitro and in a rat model of liver fibrosis

Table 1 Primer pairs for PCR and RT-RCR reactions

RIC-UP1: 5'-CATGCATTCAGCTATCCTGGCCACCTTCTTCTTGTTG-3'

RIC-DP1: 5'-CTCCATAGATGAACTCCCC-3'

RIC-UP2: 5'-CCACCATGCATTCAGCTATCCTGG-3'

RIC-DP2: 5'-GTTACTTATCATCGTCGTCCTTGTAGTCACACCACAATAAGGAATTC-3'

RIC-UP3: 5'-GAATCCAGTCTCTCTATGGTCCAG-3' (785-808)

RIC-DP3: 5'-TCATCGTCGTCCTTGTAGTCACAC-3' (1413-1436)

IC-UP4: 5'-TGGAGCCCTGATGTTTCCCATCTA--3' (693-717)

IC-DP4: 5'-ACCCAAATACTCTTTGGGAGATAA--3' (1423-1446)

β-UP5: 5'-AACCGCGAGAAGATGAACCAGATCATGTTT-3' (2135-2164)

β-DP5: 5'-AGCAGCCGTGGCCATCTCTTGCTCGAAGTC-3' (2456-2485)

( ): binding location of primer in relative cDNA sequence

Table 2 Effects of in vivo transfection of FLAG-tagged rat collagenase on the accumulation of collagen type I and III in experimental liver fibrosis

Group	n	ILD of collagen I	ILD of collagen III
A	6	17.35 ± 2.62	18.94 ± 3.78
B	6	86.83 ± 11.93a	75.21 ± 10.07a
C1	7	69.25 ± 12.31ab	62.10 ± 9.72ab
C2	7	72.18 ± 14.18ab	64.80 ± 11.69ab

^aP < 0.05 when compared with normal control group (group A);

^bP < 0.05 when compared with disease control group (group B). ILD: Integral Light Density; N: number of rats in each group