Copyright
©The Author(s) 2002.
World J Gastroenterol. Oct 15, 2002; 8(5): 792-796
Published online Oct 15, 2002. doi: 10.3748/wjg.v8.i5.792
Published online Oct 15, 2002. doi: 10.3748/wjg.v8.i5.792
Table 1 Sequences of primers for amplifed cDNA of the p53, bcl-2 and β-actin
Primers | Sequences | Combining sites | Amplifiers | |
p53 | Sense | 5'-GGAGGTTGTGAGGCGCTGC-3 | 645 bp-663 bp | 311 bp |
Antisense | 5'-CACGCACCTCAAAGCTGTTC-3 | 936 bp-955 bp | ||
bcl-2 | Sense | 5'-CAGCTGCACCTGACGCCCTT-3' | 1810 bp-1829 bp | 191 bp |
Antisense | 5'-GCCTCCGTTATCCTGGATCC-3' | 2021 bp-2040 bp | ||
β-actin | Sense | 5'-AGCGGGAAATCGTGCGTGAC-3 | 665 bp-674 bp | 471 bp |
Antisense | 5'-ACTCCTGCTTGCTGATCCACATC-3 | 1103 bp-1125 bp |
Table 2 Chinese Jianpi herbs-induced effects on gastric cancer cell SGC-7901 (¯x ± s)
Treatment | Tumor weight/g (n) | Tumor size/mm3 (n) | Percentage of control/% | Apoptotic index (AI)/% | ki-67/% | |
TUNEL (n) | FACScan (n) | |||||
Sijunzi Decoction | 0.66 ± 0.16 (9)a | 371.81 ± 52.51 (9)a | 34.33 | 16.24 ± 3.21 (9)b | 11.38 ± 6.46 (9)a | 7.95 ± 2.54 (9)c |
SRRS | 0.54 ± 0.23 (9)b | 322.66 ± 126.14 (9)b | 46.53 | 13.18 ± 3.05 (8)a | 11.58 ± 5.71 (8)a | 8.43 ± 2.22 (8)b |
Control | 1.01 ± 0.32 (10) | 603.61 ± 263.39 (10) | 2.63 ± 1.03 (10) | 7.51 ± 1.32 (10) | 10.37 ± 4.91 (10) |
Table 3 Chinese Jianpi herbs-induced genetic effects (¯x ± s)
- Citation: Zhao AG, Zhao HL, Jin XJ, Yang JK, Tang LD. Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice. World J Gastroenterol 2002; 8(5): 792-796
- URL: https://www.wjgnet.com/1007-9327/full/v8/i5/792.htm
- DOI: https://dx.doi.org/10.3748/wjg.v8.i5.792