Antitumor activities of human autologous cytokine-induced killer (CIK) cells against hepatocellular carcinoma cells in vitro and in vivo

Table 1 Phenotype of CIK cells and cytotoxic activity of CIK subsets

Subset	Percentage positively of CIK	LU/106 cell stained cells	Cell number × 106	Total LU per culture
CIK		43.6 ± 4.8	712 ± 24.3	29, 074
TCR-α/β	82.0 ± 6.4	20.6 ± 3.8	583 ± 41.6	12, 088
CD56	30.4 ± 5.6	78.4 ± 6.9	214 ± 70.3	13, 948
CD16	15.8 ± 5.4	60.4 ± 6.0	89 ± 40.9	4106

Note: Total LU per culture was calculated by multiplying the number of LU per million cells by the total number of cells.

Table 2 Inhibitory effects of CIK, PBMC and LAK cells on HCC cells in tumor-bearing nude mice

Group	n	Tumor/g	Tumorgenesis (%)	Inhibitory rate (%)
Control	9	2.10 ± 0.41	100	0
PBMC	11	1.32 ± 0.27	100	37.1
LAK	11	0.99 ± 0.33	100	52.8
CIK	15	0.32 ± 0.31	66.7	84.7ab

^aP < 0.05 vs LAK group,

^bP < 0.01 vs PBMC group.