Effect of cis-9, trans-11-conjugated linoleic acid on cell cycle of gastric adenocarcinoma cell line (SGC-7901)

Table 1 Inhibitory effect of c9,t11-CLA on DNA synthesis in SGC-7901 cells (n = 6)

c9,t11-CLA (μmol/L)	3H-TdR incorporation (cpm, ¯x ± s)		Inhibitory Rate (%)
	24 h	48 h	24 h	48 h
0	2165 ± 172	3598 ± 603	－	－
25	1810 ± 505	3093 ± 323	16.4	14.0
50	2208 ± 291	2640 ± 607a	-2.0	26.6
100	2065 ± 261	2063 ± 495b	4.6	42.7
200	472 ± 260b	88 ± 15b	78.2	97.5

^aP < 0.05,

^bP < 0.01 vs negative control

Table 2 Positive rates of cyclin A, B₁, and D₁ on SGC-7901 cells treated with c9,t11-CLA (%)

c9,t11-CLA (μmol/L)	24 h			48 h
	Cylin A	Cylin B1	Cyclin D1	Cylin A	Cylin B1	Cyclin D1
0	10.7	4.2	9.5	5.9	5.1	6.0
25	11.0	4.8	3.6b	8.5	5.5	3.7b
50	7.9	2.5	3.5b	5.0	3.1b	3.7b
100	4.4b	2.6b	2.1b	1.3b	0.7b	0.6b
200	2.3b	1.8b	0.4b	0.5b	0.6b	0

^bP < 0.01 vs negative control

Table 3 Positive rates of p16^ink4a and p21^waf1 on SGC-7901 cells treated with c9,t11-CLA (%)

c9,t11-CLA (μmol/L)	24 h		48 h
	p16ink4a	p21waf1	p16ink4a	p21waf1
0	1.0	0.2	0.8	0.6
25	0.7	1.4b	0.2	0.8
50	1.4	1.0b	3.0b	2.5b
100	2.8b	4.1b	4.6b	3.8b
200	3.6b	5.2b	5.0b	6.3b

^bP < 0.01 vs negative control