Influence of L-methionine-deprived total parenteral nutrition with 5-fluorouracil on gastric cancer and host metabolism

Table 1 Composition and dosage of TPN solution (kg¯¹•d¯¹)

Composition	+MetTPN	-MetTPN
250 g•L¯¹ Glucose/m	100	100
200 g•L¯¹ Intralipid/mL	35	35
+Met amino acid solution/mL	100	0
-Met amino acid solution/mL	0	100
Non-protein energy/J	710	710
Total nitrogen/g	1.30	1.03
Total volume/mL	235	235

Table 2 Body mass, tumor diameter and DNA index of tumor cells after TPN (mean ± SD)

TPN solution	mb/g		dt/mm		DNA index
	Pre-TPN	Post-TPN	Pre-TPN	Post-TPN	Pre-TPN	Post-TPN
+MetTPN	242 ± 20	243 ± 20	11.2 ± 1.0	14.7 ± 0.7b	1.13 ± 0.24	1.21 ± 0.26
-MetTPN	248 ± 20	245 ± 18	11.5 ± 1.2	6.9 ± 0.8ad	1.15 ± 0.21	1.12 ± 0.23
+MetTPN+5-FU	245 ± 13	233 ± 12b	11.1 ± 1.1	9.3 ± 0.8a	1.15 ± 0.03	1.08 ± 0.02a
-MetTPN+5-FU	245 ± 14	225 ± 13b	11.4 ± 0.9	5.5 ± 0.5bc	1.15 ± 0.03	1.02 ± 0.02bc

^dP ＜ 0.05,

^bP ＜ 0.01, vs preTPN;

^cP ＜ 0.05, ^dP ＜ 0.01, vs +MetTPN.

Table 3 Change of tumor cell cycles after TPN (mean ± SD, %)

TPN solution	S		G2M		G0/G1
	Pre-TPN	Post-TPN	Pre-TPN	Post-TPN	Pre-TPN	Post-TPN
+MetTPN	6.2 ± 1.2	10.3 ± 1.4b	10.5 ± 1.1	16.6 ± 1.4a	83.4 ± 1.9	73.1 ± 1.8b
-MetTPN	6.4 ± 1.0	28.5 ± 1.0ac	11.2 ± 1.1	8.3 ± 1.5ad	82.4 ± 0.9	63.2 ± 5.5a
+MetTPN+5-FU	6.6 ± 0.3	4.5 ± 0.5a	12.6 ± 0.5	9.7 ± 1.2a	80.8 ± 0.3	85.8 ± 1.2b
-MetTPN+5-FU	6.5 ± 0.7	4.5 ± 0.3a	12.4 ± 0.9	6.2 ± 0.5bc	80.1 ± 0.5	89.3 ± 0.7b

^aP ＜ 0.05,

^bP ＜ 0.01, vs preTPN;

^cP ＜ 0.05,

^dP ＜ 0.01, vs +MetTPN.

Table 4 Serum FAA value (mean ± SD, μmol•L¯¹)

	+MetTPN	-MetTPN
Asp	36.1 ± 1.2	88.9 ± 10.3a
Glu	27.3 ± 4.2	193.2 ± 17.4a
Ser	124.8 ± 21.5	231.5 ± 32.3a
Gly	116.9 ± 18.3	286.7 ± 21.9a
Gln	103.4 ± 14.4	90.1 ± 10.3
His	11.2 ± 1.5	25.0 ± 3.6
Thr	53.5 ± 3.8	93.2 ± 7.1a
Ala	138.7 ± 30.1	218.8 ± 31.4a
Arg	83.6 ± 9.4	173.4 ± 25.1a
Pro	33.9 ± 8.2	79.7 ± 13.4
Tyr	20.8 ± 5.1	44.8 ± 6.3
Val	88.4 ± 14.1	31.6 ± 1.6
Met	76.1 ± 1.3	10.9 ± 3.1b
Cys	87.3 ± 3.2	43.2 ± 5.4b
Ile	5.4 ± 1.1	10.1 ± 1.2
Leu	34.2 ± 1.3	55.2 ± 4.3
Phe	22.1 ± 2.2	43.1 ± 2.9
Trp	42.5 ± 3.6	50.7 ± 4.4
Lys	212.4 ± 43.1	387.8 ± 58.3a

^aP ＜ 0.05,

^bP ＜ 0.001, vs +MetTPN.