Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

Table 1 Tumor growth of paired fresh (F) and DMSO-frozen (D) tissue: transplanted with original tumors and xenotransplanted tumors^a

Type	TG/total (n)		Success rate (%)
	F	D	F	D
Colon	3/3 (12/12)	0/3 (6/10)b	100 (100)	0 (60)
Pancreas	2/5 (8/8)	0/5 (1/8)	40 (100)	0 (13)
Total	5/8 (20/20)	0/8 (7/18)	63 (100)	0 (39)

^aFigures in () is the results of transplantation with xenotransplanted tumors;

^b1 mouse died after transplantation.

Table 2 Influence of tumor cell content on tumor growth of fresh surgical specimens

Tumor	TG/total (n)		Success rate
	≥ 50%	< 50%	≥ 50%	< 50%
Colon	3/3	1/1	100	100
Pancreas	3/3	1/4	100	25
Total	6/6	2/5	100	40

Table 3 Serum CEA levels of mice with or without tumor

	With tumor (-x±s, mg·L-1)	Without tumor (-x±s, mg·L-1)	P value
Colon	19.33 ± 28.77 (n = 17)	0.1 ± 0.06 (n = 7)	< 0.005
Pancreas	2.85 ± 1.89 (n = 11)	0.29 ± 0.2 (n = 7)	< 0.005