Copyright
©The Author(s) 2000.
World J Gastroenterol. Jun 15, 2000; 6(3): 371-373
Published online Jun 15, 2000. doi: 10.3748/wjg.v6.i3.371
Published online Jun 15, 2000. doi: 10.3748/wjg.v6.i3.371
Table 1 Data on the synthesis of A chain analogues
| A Chain analogues | FmocAla-resinmg (mmol) | Target peptide-resin (mg) | After HPLC (mg) | Overall yield (%) |
| 1 | 500 (0.19) | 769 | 62.1 | 12.3 |
| 2 | 500 (0.19) | 749 | 54.6 | 12.0 |
| 3 | 500 (0.19) | 724 | 45.4 | 12.4 |
| 4 | 500 (0.32) | 865 | 20.0 | 4.5 |
| 5 | 300 (0.135) | 657 | 39.6 | 10.6 |
| 6 | 300 (0.135) | 648 | 40.1 | 11.2 |
| 7 | 300 (0.135) | 660 | 41.3 | 11.1 |
Table 2 Biological activities of insulin analogues (%)
| Sample | Receptor binding | Mouse convulsion | Immunological activity |
| Ins | 100 | 100 | 100 |
| 1B | 70.3 | 60 | |
| 2B | 7.9 | 6.2 | |
| 3B | 4.0 | 3.3 | |
| 4B | 10.4 | 10 | |
| 5B | 51.9 | 50 | 28.8 |
| 6B | 44.3 | 40 | 29.6 |
| 7B | 33.1 | 30 | 15.4 |
- Citation: Yang SZ, Huang YD, Jie XF, Feng YM, Niu JY. Relationship between insulin A chain regions and insulin biological activities. World J Gastroenterol 2000; 6(3): 371-373
- URL: https://www.wjgnet.com/1007-9327/full/v6/i3/371.htm
- DOI: https://dx.doi.org/10.3748/wjg.v6.i3.371