Effects of salvianolic acid-A on NIH/3T3 fibroblast proliferation, collagen synthesis and gene expression

Table 1 PCR primer sequences and expected size of amplified products

Primers	Sequence	Size
α 2(I) collagen upstream	5'TGTTCGTGGTTCTCAGGGTAG3'
α 2(I) collagen downstream	5'TTGTCGTAGCAGGGTTCTTTC3'	254 bp
β-actin upstream	5'ACATCTGCTGGAAGGTGGAC3'
β-actin downstream	5'GGTACCACCATGTACCCAGG3'	163 bp

Table 2 Effects of SA-A on cell intracellular [³H]TdR and [³H]Pro incorporation (cpm/well, ^-x±s, n = 4)

Group	[3H]TdR	[3H]Pro
Control	1482 ± 486	21018 ± 5473
10-4 mol/L SA-A	675 ± 201b	18659 ± 2363
10-5 mol/L SA-A	969 ± 183a	23761 ± 5430
10-6 mol/L SA-A	868 ± 183a	31408 ± 4981a
10-7 mol/L SA-A	1056 ± 187	26080 ± 4504

^aP < 0.05,

^bP < 0.01, vs control.

Table 3 Effects of SA-A on NIH/3T3 fibroblast collagen synthetic rates (%,^-x±s, n = 4)

Group	Intracellular	Extracellular
Control	0.78 ± 0.03	2.57 ± 0.37
10-5 mol/L	0.48 ± 0.24b	2.54 ± 0.91
10-6 mol/L	0.43 ± 0.26b	3.02 ± 0.69

^bP < 0.01, vs control.

Table 4 The relative expression amount of α2(I) procollagen mRNA (^-x±s,% of β-actin)

Group	n	Col α1(I) mRNA
Control	3	98.71 ± 9.96
10-5 mol/L SA-A	3	76.23 ± 12.02a
10-6 mol/L SA-A	3	68.44 ± 8.06a

^aP < 0.05, vs control.