Copyright
©The Author(s) 2000.
World J Gastroenterol. Apr 15, 2000; 6(2): 202-209
Published online Apr 15, 2000. doi: 10.3748/wjg.v6.i2.202
Published online Apr 15, 2000. doi: 10.3748/wjg.v6.i2.202
Table 1 Major sugar specificities of lectins used in this study
Lectin | Abbreviation | Carbohydrate specificity |
Arachis hypogaea | PNA | β-D-Gal (1-3)GalNAc |
Bandeirae simplicifolia | BS-I | α-Gal, α-GalNAc |
Concanavalin A | Con A | α-D-Man, α-D-Glc |
Datura stramonium | DSA | (D-GlcNAc)2 |
Glycine max | SBA | D-GalNAc |
Helix promatia | HPA | D-GalNAc |
Lens culinaris | LcH | α-D-Man |
Narcissus pseudonarcissus | NPA | α-D-Man |
Pisum sativum | PEA | α-D-Man |
Tetragonolobus purpureas | Lotus A | α-L-Fuc |
Triticum vulgaris | WGA | (D-GlcNAc)2, NeuNAc |
Vicia faba | VFA | D-Man, D-Glc |
Vicia sativa | VSA | D-Glc, D-Man |
Vicia villosa | VVA | D-GalNAc |
Vigna radiata | MBA | α-Gal |
Table 2 Lectin agglutination patterns of H. pylori coccoids and spirals
Lectin | RH 54 | NCTC 11637 | V2 | |||
Coccoids | Spirals | Coccoids | Spirals | Coccoids | Spirals | |
Con A | 2 | 1 | 3 | 2 | 2 | 1 |
LcH | 2 | 0 | 2 | 0 | 1 | 0 |
NPA | 1 | 0 | 1 | 0 | 1 | 0 |
PEA | 2 | 1 | 2 | W | 1 | 1 |
VFA | 1 | 1 | 2 | 1 | 1 | 1 |
Lotus A | 3 | 3 | 1 | 0 | 3 | 3 |
DSA | 1 | 2 | 1 | 0 | 1 | 2 |
WGA | 2 | 3 | 0 | 0 | 2 | 3 |
HPA | 1 | 2 | 1 | 1 | 1 | 1 |
SBA | 1 | 1 | 1 | 1 | 0 | 3 |
VVA | 1 | 1 | 0 | 1 | 0 | 1 |
VSA | 1 | 1 | 2 | 1 | 2 | 1 |
PNA | 1 | 1 | 1 | 0 | 1 | 2 |
BS-I | 2 | 2 | 1 | 0 | 1 | 2 |
MBA | 1 | 3 | 1 | 1 | 0 | 1 |
Sugar specificity/Lectins | ||||||
Man- | Con A = Concanavalin A; | |||||
NPA = Narcissus pseudonarcissus; | ||||||
PEA = Pisum sativum; | ||||||
VFA = Vicia faba | ||||||
Fuc- | Lotus A = Tetragonolobus purpureas; | |||||
(GlcNAc)2- | DSA = Datura stramonium; | |||||
WGA = Triticum vulgaris | ||||||
GalNAc- | HPA = Helix promatia; | |||||
SBA = Glycine max; | ||||||
VVA = Vicia villosa. | ||||||
Glc- | VSA = Vicia sativa | |||||
Glc- | PNA = Arachis hypogaea; | |||||
BS-I = Bandeirae simplicifolia | ||||||
MBA = Vigna radiata |
Table 3 Strains of H. pylori agglutinated by lectins of various specificities
Sugar-specificity/Lectin | No. of H. pylori strains agglutinated | |
Coccoids | Spirals | |
α-D-Man, α-D-Glc/Con A | 3 | 3 |
α-D-Man/LcH | 3 | 0 |
α-D-Man/NPA | 3 | 0 |
α-D-Man/PEA | 3 | 3 |
D-Man, D-Glc/VFA | 3 | 3 |
α-L-Fuc/Lotus A | 3 | 2 |
(D-GlcNAc)2/DSA | 3 | 2 |
(D-GlcNAc)2/WGA | 2 | 2 |
D-GalNAc/HPA | 3 | 3 |
D-GalNAc/SBA | 2 | 3 |
D-GalNAc/VVA | 1 | 3 |
D-Glc, D-Man/VSA | 3 | 3 |
β-D-Gal (1-3)GalNAc/PNA | 3 | 2 |
α-Gal, α-GalNAc/BS-I | 3 | 2 |
α-Gal/MBA | 2 | 3 |
Table 4 H. pylori lectin-binding inhibition
Inhibitor | RH 54 coccoids | RH 54 spirals | ||||
Con A | Lotus A | WGA | Con A | Lotus A | WGA | |
Glycoproteins | ||||||
Bovine submaxillary mucin | 1 | 3 | 2 | 0 | 3 | 3 |
Porcine stomach mucin | 1 | 2 | 0 | 1 | 3 | 0 |
Fetuin | 2 | 3 | W | W | 3 | 3 |
Asialofetuin | 1 | 3 | 1 | W | 3 | 3 |
Bovine orosomucoid | 1 | 3 | W | 1 | 3 | 2 |
Gangliosides II | 1 | 3 | W | 0 | 3 | 2 |
Carbohydrates | ||||||
Gal | 1 | 3 | 1 | W | 3 | 3 |
GalNAc | 1 | 3 | 1 | W | 3 | 3 |
GlcNAc | W | 3 | 1 | 0 | 3 | 3 |
NANA | 1 | 2 | 1 | 1 | 3 | 3 |
Man | 0 | 3 | 1 | 0 | 3 | 3 |
Glc | W | 3 | 1 | 0 | 3 | 3 |
Malt | 0 | 3 | 1 | 0 | 3 | 3 |
Suc | W | 3 | 1 | 0 | 3 | 3 |
Fuc | 1 | 0 | 2 | 0 | 0 | 3 |
Control | 2 | 3 | 2 | 1 | 3 | 3 |
Table 5 Effect of chemical and enzymatic treatments on H. pylori lectin binding
Inhibitor | RH 54 coccoids | RH 54 spirals | ||||
Con A | Lotus A | WGA | Con A | Lotus A | WGA | |
NAIO4 (0.075 M) | W | 0 | 0 | W | 0 | 0 |
H2SO4 (0.025 N) | 1 | 3 | 2 | 1 | 3 | 3 |
Formalin (1.0%) | 2 | 3 | 2 | 1 | 3 | 3 |
Glycine HCl buffer (pH 2.2) | 1 | 3 | 2 | 1 | 3 | 3 |
60 °C (1 h) | 3 | 3 | 3 | 3 | 3 | 3 |
Neuraminidase | 1 | 3 | 2 | 1 | 3 | 3 |
Trypsin | 1 | 3 | 2 | 1 | 3 | 3 |
Pepsin | 1 | 3 | 2 | 1 | 3 | 3 |
Proteinase K | 1 | 3 | 2 | 1 | 3 | 3 |
Chymotrypsin | 1 | 3 | 2 | 1 | 3 | 3 |
Control | 2 | 3 | 2 | 1 | 3 | 3 |
- Citation: Khin MM, Hua JS, Ng HC, Wadström T, Ho B. Agglutination of Helicobacter pylori coccoids by lectins. World J Gastroenterol 2000; 6(2): 202-209
- URL: https://www.wjgnet.com/1007-9327/full/v6/i2/202.htm
- DOI: https://dx.doi.org/10.3748/wjg.v6.i2.202