Effect of cholecystokinin and secretin on contractile activity of isolated gastric muscle strips in guinea pigs

Table 1 Effects of CCK-8 on the resting tension of gastric strips in guinea pigs

Part of stomach		CCK-8	CCK-8 + secretin	Atropine and CCK-8		Indomethacin and CCK-8
	LM	19.7 ± 2.1b	23.1 ± 2.1b	-25.1 ± 4.0b	47.1 ± 6.0b	-34.5 ± 4.4b	37.6 ± 5.6b
Fundus		(n = 19)	(n = 17)	(n = 17)	(n = 17)	(n = 17)	(n = 17)
	CM	16.7 ± 2.2b	16.0 ± 1.8b	-14.9 ± 2.5b	35.2 ± 6.5b	-14.1 ± 3.2b	30.3 ± 7.0b
		(n = 16)	(n = 15)	(n = 17)	(n = 17)	(n = 17)	(n = 17)
	LM	16.8 ± 2.3b	20.4 ± 1.9b	-21.7 ± 3.4b	18.3 ± 2.8b	-8.3 ± 3.1a	13.0 ± 1.7b
Body		(n = 19)	(n = 17)	(n = 17)	(n = 17)	(n = 16)	(n = 16)
	CM	12.7 ± 2.6b	15.9 ± 3.3b	-6.7 ± 1.8b	18.3 ± 2.4b	-5.7 ± 1.8a	12.3 ± 2.1b
		(n = 11)	(n = 13)	(n = 15)	(n = 15)	(n = 14)	(n = 14)
Antrum	LM	12.3 ± 1.3b	11.6 ± 2.0b	-2.9 ± 1.2a	8.6 ± 1.7b	-1.9 ± 0.8a	4.9 ± 0.9b
		(n = 13)	(n = 15)	(n = 14)	(n = 14)	(n = 14)	(n = 14)
	CM	16.7 ± 4.5b	28.4 ± 4.1b	0	23.8 ± 4.1b	5.0 ± 5.0	30.0 ± 4.8b
		(n = 11)	(n = 17)	(n = 14)	(n = 14)	(n = 16)	(n = 16)
Pylorus	CM	12.7 ± 5.0a	8.9 ± 3.1a	0	8.2 ± 2.4a	0	8.0 ± 3.4a
		(n = 10)	(n = 12)	(n = 13)	(n = 13)	(n = 10)	(n = 10)

L(C)M, longitudinal (circular) muscle; dose: CCK^-8 10^-2μmol/L, secretin 10^-2μmol/L; atropine 10^-2 mmol/L, indomethacin 10^-2 mmol/L;

^aP < 0.05,

^bP < 0.01 vs control; 0, no effect; ^-x ± s_x% increase.

Table 2 Effects of CCK-8 on the mean contractile amplitude of body and antrum, and the motility index of pylorus in guinea pigs

Part of stomach		CCK-8	CCK-8 + secretin	Atropine and CCK-8		Indomethacin and CCK-8
Body	LM	-10.3 ± 3.3a	-8.4 ± 3.4a	-17.1 ± 4.8b	1.5 ± 2.1	-8.6 ± 2.7a	-9.7 ± 3.3a
		(n = 19)	(n = 17)	(n = 17)	(n=17)	(n=16)	(n = 16)
	CM	-2.3 ± 2.3	0	-23.3 ± 6.6b	0	0	0
		(n = 11)	(n = 13)	(n = 15)	(n = 15)	(n = 14)	(n = 14)
Antrum	LM	-10.5 ± 4.6a	-9.9 ± 4.3a	-22.2 ± 5.9b	-12.3 ± 4.8a	-8.2 ± 2.6a	-10.0 ± 2.9a
		(n = 13)	(n = 15)	(n = 14)	(n = 14)	(n = 14)	(n = 14)
	CM	58.6 ± 18.1a	48.8 ± 10.8b	-15.7 ± 5.4a	49.8 ± 11.6b	0	45.6 ± 6.6b
		(n = 11)	(n = 17)	(n = 14)	(n = 14)	(n = 16)	(n = 16)
Pylorus	CM	145.0 ± 23.8a	162.5 ± 31.7a	-41.9 ± 12.2a	161.5 ± 55.0a	0	140.0 ± 21.9b
		(n = 10)	(n = 12)	(n = 13)	(n = 13)	(n = 10)	(n = 10)

L(C)M, longitudinal (circular) muscle; dose: CCK-8 10^-2μmol/L, secretin 10^-2μmol/L; atropine 10^-2 mmol/L, indomethacin 10^-2 mmol/ L;

^aP < 0.05,

^bP < 0.01 vs control; 0, no effect; ^-x ± s_x% increase.