Tracing method study of bacterial translocation in vivo

Table 1 Detection rate of fluorescence-labeling bacteria

Organ and tissue	No. of samples	Positive rate (%)	No. of FB/* 10 × 100
Mesenteric lymph nodes	40	38(95.0)	3.8
Liver	40	23(57.5)	2.1
Subeschar	40	13(32.5)	1.1

Table 2 Quantity and rates at different periods after burn

Group	t/h	Mesenteric lymph node		Liver
		Rate	Quantity(cfu/g)	Rate	Quantity(cfu/g)
Burn	6	100	3.0 × 104	70	2.1 × 104
	12	100	8.5 × 104	70	3.1 × 103
	24	90	7.4 × 104	50	2.6 × 103
	48	70	8.2 × 103	40	1.5 × 103
Normal	6	40	8.0 × 103	0	0

Table 3 Quantity and positive rate of PUC19 bacteria after burn

Group	t/h	Mesenteric lymph node		Liver
		Quantity(cfu/g)	Rate(%)	Quantity(cfu/g)	Rate(%)
	6	5.0 × 104	89.	2.3 × 103	60.
	12	4.0 × 104	80.	2.4 × 103	60.
Burn	24	3.1 × 104	80.	2.6 × 103	50.
	48	8.0 × 103	62.	8.0 × 103	37.
	12(d)	5.1 × 103	25.	0	0
	6	1.0 × 105	20.	0	0

Table 4 Comparison of positive rates of PUC19 in early and late stages postburn

Organ	Early stage within 48 h postburn		Late stage 12 d postburn
	No. of samples	Positive(%)	No. of samples	Positive(%)
Mesenteric lymph nodes37		29(78.4)	8	1(12.5)
Liver	37	19(51.4)	8	0(0.0)
Subeschar	37	4(10.8)	8	5(62.5)