Dynamic and ultrastructural study of sphincter of Oddi in early-stage cholelithiasis in rabbits with hypercholesterolemia

Table 1 Serum cholesterol concentration (mmol/L)

Group	Pre-feeding	Post-feeding
Control	1.43 ± 0.61	1.36 ± 0.42
Group I	1.57 ± 0.56a	30.61 ± 6.32a
Group II	1.40 ± 0.68b	29.03 ± 2.59b

Post

^aP < 0.01, t = 13.06,

^bP < 0.01, t = 32.17 vs control.

Table 2 Manometry of rabbits SO (mmHg)

	Proximal segment		HPZ
	Basal pressure	Amplitude	Basal pressure	Amplitude
Controls	11.7 ± 2.8	18.1 ± 5.9	69.3 ± 9.8	8.7 ± 3.6
Group I	20.9 ± 6.1	6.4 ± 4.5a	138.4 ± 45.5	4.2 ± 2.2a
Group II	25.6 ± 9.1	6.7 ± 4.8b	144.5 ± 40.5	3.4 ± 2.2b

Proximal: Basal pressure

^aP < 0.01, t = 4.20,

^bP < 0.01, t = 5.11 vs controls; Amplitude ^aP < 0.01, t = 4.47, ^bP < 0.01, t = 4. 24 vs control. HPZ: Basal pressure ^aP < 0.01, t = 4.20, ^bP < 0.01, t = 5.11 vs control; Amplitude ^aP < 0.01, t = 3.15, ^bP < 0.01, t = 3.17 vs control.

Table 3 Rabbit’s smooth muscle of SO NADPH stain imaging analysis (μm)

	Total (number)	Total (area)	Area (density)	Area (percentage)	Mean (string)	Mean (spatial range)
Controls	107.6 ± 64.4	330.5 ± 133.4	0.0079 ± 0.0048	2.4 ± 1.0	1.6 ± 0.4	77.2 ± 67.7
Group I	117.6 ± 43.4	319.5 ± 126.5	0.0087 ± 0.0032	2.4 ± 0.9	1.5 ± 0.3	69.7 ± 26.2
Group II	109.6 ± 45.4	360.4 ± 190.4	0.0081 ± 0.0033	2.7 ± 1.4	1.6 ± 0.4	79.9 ± 61.7

Reference area is 13550.6 μm.