Study on the anticarcinogenic effect and acute toxicity of liver-targeting mitoxantrone nanoparticles

Table 1 Weight (g) of tumor taken from four different groups

Group	Liver tumor	Armpit tumor
DHAQ-PBCA-NP
1	0.000	0.215
2	0.000	0.060
3	0.000	0.005
4	0.000	0.015
5	0.030	0.015
ADR
1	0.820	0.100
2	0.000	0.050
3	0.675	0.137
4	0.210	0.410
DHAQ
1	0.585	0.270
2	0.650	0.120
3	0.000	0.065
4	0.000	0.082
5	0.500	0.066
0.9% NS
1	1.320	1.160
2	1.530	1.026
3	1.000	0.635
4	0.420	0.670

Table 2 Rate of tumor inhibition (RTI, %) of each group of nude mice

Drug	RTI (orthotopic)	RTI (heterotopic)
DHAQ	67.49a	86.18d
ADR	60.07b	80.03e
DHAQ-PBCA-0.9%NS	99.44c	92.90f

c vs b, a P < 0.05; f vs d, e P > 0.05; b vs a P > 0.05.

Table 3 The cell proliferative activity of tumor of each nude mice

	DHAQ-PBCA-NP					DHAQ					ADR				0.9%NS
	1	2	3	4	5	1	2	3	4	5	1	2	3	4	1	2	3	4
PCNA(%)	7	2	1	3	2	7	9	7	6	9	7	9	10	82	10	95	10	90
	0	0	0	0	5	0	0	0	0	0	0	0	0		0		0
-x±s (%)	31.0 ± 23.0 a					76 ± 13.4b					85.5 ± 12.7c				96.3 ± 4.8

a vs b, c P < 0.05; b vs c P < 0.05.

Table 4 The LD₅₀ of DHAQ-PBCA-NP, DHAQ and PBCA-NP i.v. in mice

Group	n	LD50(mg/kg, P = 0.95)*
		7 d	14 d	21 d
DHAQ	60	12.8 ± 1.9	8.2 ± 1.7	6.1 ± 1.0
DHAQ-NP	60	309.9 ± 26.2	301.0 ± 28.3	299.0 ± 24.2
DHAQ-PBCA-NP	60	16.9 ± 3.9	12.3 ± 2.7	10.1 ± 1.9

*Dosage calculated as DHAQ.