Preparation and distribution of 5-fluorouracil 125I sodium alginate-bovine serum albumin nanoparticles

Table 1 Encapsulation efficiency of 5-FU in NP

Weight of drug added (g)	A value	Weight of drug encapsulated (g)	Encapsulation efficiency (%)
0.6162	0.679	0.208	33.8
0.6113	0.625	0.193	31.6
0.5917	0.676	0.195	32.9

Table 2 Rate of recovery of 5-FU

Weight of 5-FU added (g)	Weight of 5-FU determined (g)	Rate of recovery (%)
0.1348	0.1326	98.4
0.1463	0.1449	99.1
0.1429	0.1395	97.6

Table 3 Distribution of ¹²⁵I sodium alginate-BSA NP in rats (n = 3, dosage 148 × 10⁸ Bq per rat, po.)

Organ	Value of radioactive counting of 100mg tissue in 30s at different time points
	1 h	4 h	8 h	16 h	24 h	36 h
Blood	502 ± 100	601 ± 135	373 ± 22	298 ± 19	191 ± 17	129 ± 23
Heart	436 ± 110(0.86)	801 ± 66(0.33)	265 ± 23(0.71)	230 ± 23(0.77)	190 ± 10(0.99)	87 ± 35(0.67)
Liver	436 ± 110(0.86)	908 ± 100(1.51)	777 ± 69(2.08)	405 ± 97(1.36)	226 ± 16(1.18)	153 ± 21(1.19)
Spleen	663 ± 140(1.32)	1029 ± 168(1.71)	834 ± 272(2.32)	436 ± 195(1.46)	233 ± 25(1.22)	169 ± 27(0.95)
Lungs	717 ± 314(1.42)	818 ± 172(1.36)	597 ± 121(1.60)	443 ± 31(1.49)	166 ± 4(0.87)	119 ± 27(0.92)
Kidneys	526 ± 139(1.05)	185 ± 39(0.14)	401 ± 71(1.08)	387 ± 105(1.30)	133 ± 13(0.69)	123 ± 14(0.95)
Brain	105 ± 31(0.21)	112 ± 49(0.19)	111 ± 47(0.30)	96 ± 27(0.32)	50 ± 5(0.26)	39 ± 11(0.30)
Stomach	89654 ± 7143	28674 ± 3647	8362 ± 1902	4641 ± 1400	698 ± 34	527 ± 114
Intestine	765 ± 137	5573 ± 891	30 ± 599	403 ± 118	115 ± 64	204 ± 46
Bowel	1256 ± 664	639 ± 1023	30 ± 599	3221 ± 863	125 ± 60	256 ± 102

Table 4 Pharmacolinetical parameters of ¹²⁵I sodium alginate-BSA NP in rats

Parameter	Unit	Value
A	Bq	3022 × 102
α	1/h	0.07
B	Bq	3.03 × 103
β	1/h	-0.11
Ka	1/h	1.46
V/F	Bq	1428 × 103
T1/2α	h	9.42
T1/2β	h	-6.07
T1/2Ka	h	0.47
K21	1/h	-0.11
K10	1/h	0.07
K12	1/h	-0.00035
AUC	Bq × h	3.9 × 109
CL(s)	Bq × h/Bq	105 × 103
T(peak)	h	2.18
Cmax	Bq	2.45 × 107