Protective effects of polydatin against CCl4-induced injury to primarily cultured rat hepatocytes

Table 1 Effect of PD on GPT activity in culture medium (^-x±s, n = 8)

Group c/(mol/L)		GPT(U)
		6 h	12 h	24 h	48 h
Normal		13.5 ± 2.5b	13.8 ± 3.1b	13.7 ± 5.6b	14.1 ± 3.3b
Control		72.3 ± 14.1	79.7 ± 10.3	85.4 ± 9.2	88.3 ± 19.6
P D	10-7	60.3 ± 17.1a	62.0 ± 15.6a	68.8 ± 17.5a	71.4 ± 20.5a
P D	10-6	55.0 ± 10.3a	58.3 ± 16.7a	64.1 ± 13.6a	69.1 ± 19.2a
P D	10-5	30.6 ± 10.6b	38.3 ± 5.5b	42.5 ± 7.0b	45.0 ± 7.6b
P D	10-4	42.1 ± 7.8a	47.5 ± 9.8a	56.8 ± 11.3a	59.2 ± 10.7a

^aP < 0.05,

^bP < 0.01, vs CCl₄-treated control group.

Table 2 Effects of PD on GSH content in culture supernatant (^-x±s, n = 8)

Group c/(mol/L)		GSH (ng/L) after CCl4 challenge
		6 h	12 h	24 h	48 h
Normal		9.8 ± 0.8b	10.1 ± 0.8b	10.4 ± 0.7b	10.6 ± 1.2b
Control		4.2 ± 0.6	4.1 ± 0.7	4.1 ± 0.3	3.8 ± 0.6
P D	10-7	5.0 ± 0.3	5.4 ± 0.5	5.6 ± 0.9	6.1 ± 1.0a
P D	10-6	5.3 ± 0.8	5.6 ± 0.9	6.4 ± 0.6a	6.8 ± 1.1a
P D	10-5	8.4 ± 1.2b	5.9 ± 1.3a	7.7 ± 0.8a	9.0 ± 1.2b
P D	10-4	6.7 ± 0.4a	6.1 ± 1.0a	6.8 ± 0.7a	7.6 ± 0.9a

^aP < 0.05,

^bP < 0.01, vs CCl₄ treated control group.

Table 3 Effects of PD on MDA formation in rat hepatocytes (^-x±s, n = 8)

Group c/(mol/L)		GSH (ng/L) after CCl4 challenge
		6 h	12 h	24 h	48 h
Normal		4.0 ± 0.4b	4.5 ± 0.6b	4.8 ± 0.4b	4.6 ± 0.7b
Control		15.5 ± 1.8	16.0 ± 2.7	17.5 ± 2.1	19.0 ± 2.4
P D	10-7	13.1 ± 2.0	13.8 ± 3.3	13.0 ± 4.3a	14.5 ± 1.8a
P D	10-6	11.4 ± 1.7a	12.0 ± 1.8a	12.1 ± 3.1a	12.5 ± 2.0a
P D	10-5	6.5 ± 1.2b	6.7 ± 1.2b	7.5 ± 2.3b	8.2 ± 2.7b
P D	10-4	8.7 ± 3.5b	8.9 ± 2.8b	9.8 ± 2.6b	10.3 ± 3.0b

^aP < 0.05,

^bP < 0.01, vs CCl₄ treated control group.