Expression and kinetic changes of alkaline phosphatase and its isoenzymes in experimental rat hepatoma

Table 1 Results of histological examination of rat livers

Groups	n	Precancerosis	Canceration
Control group	6	0	0
2nd week	6	6	0
4nd week	6	2	4
6nd week	6	0	6
8nd week	6	0	6
10nd week	6	0	6
12nd week	6	0	6

Table 2 Kinetic changes of ALP of rat sera and livers

Groups	n	Serum ALP (U/L)	ALP of homogenate (U/g)
			B (Total ALP)	A (Soluble)	B-A (Membrane combined)	A/B-A
Control group	6	9.2 ± 4.1	16.8 ± 2.7	7.5 ± 3.5	9.3 ± 4.2	0.81
2nd week	6	22.0 ± 6.3a	57.8 ± 8.2a	24.9 ± 7.2a	32.9 ± 6.5a	0.76
4nd week	6	26.2 ± 20.9a	56.2 ± 31.7a	25.7 ± 13.9a	30.4 ± 21.1a	0.85
6nd week	6	25.0 ± 8.0a	127.5 ± 43.9a	72.1 ± 32.4a	55.4 ± 22.9a	1.30a
8nd week	6	32.8 ± 4.0a	73.1 ± 11.8a	51.2 ± 6.8a	21.9 ± 6.5a	2.34a
10nd week	6	33.3 ± 3.1a	77.6 ± 20.3a	52.1 ± 7.1a	25.5 ± 14.5a	2.04a
12nd week	6	27.0 ± 2.9a	60.5 ± 4.1a	38.0 ± 6.9a	22.5 ± 6.6a	1.69a

^aP < 0.01 compared with control group.

Table 3 Changes of ALP’s isoenzymes

Groups	n	Serum (liver tissues) ALP isoenzyme
		I	II	III	IV	V
Control group	6	0(6)	0(0)	6(6)	6(6)	6(6)
2nd week	6	0(6)	0(0)	6(6)	6(6)	6(6)
4th week	6	0(1)	0(0)	6(6)	6(6)	6(6)
6th week	6	0(0)	0(4)	6(6)	6(6)	6(6)
8th week	6	0(0)	4(6)	6(6)	6(6)	6(6)
10th week	6	0(0)	6(6)	6(6)	6(6)	6(6)
12th week	6	0(0)	6(6)	6(6)	6(6)	6(6)