Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 1998; 4(4): 323-325
Published online Aug 15, 1998. doi: 10.3748/wjg.v4.i4.323
Expression and kinetic changes of alkaline phosphatase and its isoenzymes in experimental rat hepatoma
Ming-Bing Xiao, Deng-Fu Yao, Hong Zhang, Hui Qiang, Jie-Fei Huang, Qun Wei, Feng Jiang
Ming-Bing Xiao, Deng-Fu Yao, Hong Zhang, Hui Qiang, Jie-Fei Huang, Qun Wei, Feng Jiang, Department of Gastroenterology, Affiliated Hospital, Nantong Medical College, Nantong 226001, Jiangsu Province, China
Ming-Bing Xiao, male, born on December 16, 1972 in Rudong, Jiangsu Province, graduated from Nantong Medical College, engaged in the research of early diagnosis of hepatoma, having more than 8 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Ming-Bing Xiao, Department of Gastroenterology, Affiliated Hospital, Nantong Medical College, No. 20 Xisi Road, Nantong 226001, Jiangsu Province, China
Telephone: +86-513-5119461
Received: April 1, 1998
Revised: May 12, 1998
Accepted: June 10, 1998
Published online: August 15, 1998
Abstract

AIM: To explore the expression and changes of hepatoma specific alkaline phospatase (ALP) in rats during canceration.

METHODS: The ALPs and isoenzymes of rat livers and sera were investigated in SD hepatomas induced with 0.05% 2-fluoenylacetamide (2-FAA).

RESULTS: By pathological examination and biochemical analysis. ALPs were overexpressed in rat livers during canceration and then were secreted into blood. Serum total ALP activities, liver ALP specific activities (U/g) including soluble and membrane-combined ALP activities of each group were all significantly higher (P < 0.01)than those of control group. The average ratios of soluble ALP to membrane-combined ALP were increased significantly after 6 weeks. ALP isoenzymes of rat sera and livers showed 5 bands on PAGE: ALP-I and ALP-II were specific for normal liver and rat hepatoma tissues, the ALP-II appeared in rat liver after 6 weeks and in sera after 8 weeks.

CONCLUSIONS: ALP with carcino-embryonic protein was overexpressed in hepatoma tissues; the abnormal ALP-II of ALP isoenzymes in sera and liver of rats can be used as a tumor marker for early diagnosis of rat hepatoma.

Keywords: liver neoplasms/enzymology; alkaline phosphatase/analysis; alkaline phosphatase isoenzymes/ analysis; disease models, animal; 2-fluoenylacetamide/ analysis