Relationship between HBV viremia level of pregnant women and intrauterine infection: neated PCR for detection of HBV DNA

Table 1 Sequences of primers

Number	Position	Sequence (5’-3’)
1	300-321	CATCTTCTTGTTGGTTCTTCTG
2	715-695	TTAGGGTTTAAATGTATACCC
3	421-441	TCTATGTTTCCCTCTTGTTGC
4	626-605	ACCACATCATCCATATATCTG

1, 2 outer primers; 3, 4 inner primers

Table 2 Detection rate of HBsAg and HBV DNA

Group	Cases	Mother	Newborn
		HBV DNA+ (%)	HBV DNA+ (%)	HBsAg+ (%)
A1	14	14 (100.0)	13 (92.9)	6 (42.9)
A2	27	7 (25.9)	4 (14.8)	1 (3.7)
B	12	1 (8.3)	0	0

Table 3 Relationship between HBV intrauterine infection and mothers’ HBV DNA status

Mothers’ HBV DNA	No. of neonates	HBV DNA+	Intrauterine infection rate (%)
+	22	17	77.2
-	31	0	0

^bP < 0.01 vs mothers’ HBV DNA positive

Table 4 Relationship between HBV intrauterine infection and mothers’ serum HBV DNA levels

Mothers’ HBV DNA level	No. of neonates	HBV DNA+	Intrauterine infection rate (%)
Strong positive	15	14	93.3
Weak positive	7	3	42.9

^aP < 0.05 vs mother HBV DNA weak positive.