Glial cell line-derived neurotrophic factor improves impaired colonic motility in experimental colitis mice through connexin 43

Table 1 Disease activity index scoring system

DAI score	Weight loss (%)	Stool condition	Gross bleeding
0	None	Normal	None
1	1-5
2	5-10	Loose stools	Hemoccult positive
3	10-20
4	> 20	Diarrhea	Severe bleeding

DAI: Disease activity index.

Table 2 Histological scoring criteria

Score	Tissue damage	Lamina propria inflammatory cell infiltration
0	None	Infrequent
1	Isolated focal epithelial damage	Increased, some neutrophils
2	Mucosal erosions and ulcerations	Submucosal presence of inflammatory cell clusters
3	Extensive damage deep into the bowel wall	Transmural cell infiltrations

Table 3 Primers for quantitative real-time polymerase chain reaction analysis

Primers
GDNF	Forward	5’-GTTAATGTCCAACTGGGGGTCTA-3’
GDNF	Reverse	5’-ACAGCCACGACATCCCATAACT-3’
Cx43	Forward	5’-GGGTGATGAACAGTCTGCCTTT-3’
Cx43	Reverse	5’-AGCTTCTCTTCCTTTCTCATCACAT-3’
GAPDH	Forward	5’-CCTCGTCCCGTAGACAAAATG-3’
GAPDH	Reverse	5’-TGAGGTCAATGAAGGGGTCGT-3’

GDNF: Glial cell line-derived neurotrophic factor; Cx43: Connexin 43; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.