Loss of monopolar spindle-binding protein 3B expression promotes colorectal cancer invasiveness by activation of target of rapamycin kinase/autophagy signaling

Table 1 Primer sequences for polymerase chain reaction

Gene	DNA sequence
MOB3B	5’-CGGTGGCAGGATGATCTCAAGTATAAG-3’
	5’-CGTTGTTGATCTGAACCTCAATCCAATC-3’
GAPDH	5’-GCACCGTCAAGGCTGAGAAC-3’
	5’-TGGTGAAGACGCCAGTGGA-3’

MOB3B: Monopolar spindle-binding protein 3B.

Table 2 Downregulation of monopolar spindle-binding protein 3B expression in colorectal cancer vs normal tissues, n (%)

MOB3B protein	CRC, n = 102	Normal tissues, n = 60	P value
Negative (-)	53 (52.0)	16 (26.7)	0.002a
Positive (+)	49 (48.0)	44 (73.3)

^aP < 0.05, χ² test.

MOB3B: Monopolar spindle-binding protein 3B; CRC: Colorectal cancer.

Table 3 Association of monopolar spindle-binding protein 3B expression with clinicopathological characteristics of colorectal cancer patients

Clinicopathological characteristic	MOB3B		P value
	+	-
Sex			0.12
Male	28	38
Female	21	15
Age (years)			0.53
≥ 60	21	26
< 60	28	27
Tumor size (cm)			0.35
≥ 5.0	27	34
< 5.0	22	19
Lymph node metastasis			< 0.001a
N0	41	22
N1 + N2	8	31
Distant metastasis			0.001a
M0	48	40
M1	1	13
Tumor-node-metastasis stage			< 0.001a
I + II	41	18
III + IV	8	35

^aP < 0.05, χ² test.

MOB3B: Monopolar spindle-binding protein 3B.